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Attune™ NxT Flow Cytometer, blue/yellow

Product Code. 17133049
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Product Code. 17133049 Supplier Invitrogen Supplier No. A24861CFR

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Description

Designed and developed to remove common barriers associated with flow cytometry

  • The 2019 Attune NxT Flow Cytometer has expanded functionalities to drive your research forward. Designed and developed to remove common barriers associated with flow cytometry, the evolutionary capabilities of the Attune NxT flow cytometer offer adaptable optical configuration options so you can get the most out of your multicolor analysis.
  • Free Attune NxT software with researcher-inspired upgrades
  • New green laser option for expanded utility and fluorochrome options
  • Simple push-button sample recovery function
  • The innovative combination of acoustic focusing technology coupled with traditional hydrodynamic focusing results in acquisition speeds up to 10X faster than other flow cytometers. This technology reduces the time needed to obtain results.
  • True 35,000 event/sec (+10% coincidence rates)
  • Maximum electronic speed of 65,000 events/sec
  • Analyze small or limited samples faster than ever
  • Efficiently run traditionally long assays
  • The Attune NxT flow cytometer is ideal for immunophenotyping and signaling studies, cell cycle analysis, detection of rare events, stem cell analysis, cancer and apoptosis studies, microbiological assays, and more.
  • The instrument’s modular design provides the flexibility to meet future capability needs with a variety of upgrade options. Purchase what you need now and easily upgrade later. The small footprint of the instrument requires minimal bench space, even allowing use in a cell culture hood.
  • Flexibility—1 to 4 lasers, 14 colors, and 16 parameters
  • Field upgradeable—choose to add additional lasers when you decide
  • Customizable band pass filters for expanded fluorochrome choices
  • The Attune NxT Flow Cytometer easily runs most samples including cardiomyocytes. The system is designed with a clog-resistant syringe-driven system to push the sample with a force of up to 75 psi.
  • Flexibility in sample concentrations and sample types
  • Elimination of time-consuming concentration steps with No-Wash, No-Lyse Filter Kit
  • Analysis of dilute samples, including CSF, stem cells, mouse bone marrow, mouse blood, and thin needle aspirates
  • Acquisition of a high volume of samples in minimal time
TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Attune™ Acoustic Focusing Cytometer
Minitower Windows™ 7 64-bit computer with 23-inch flat panel monitor
Power cord with adapters
USB cable
User manual document CD
Attune™ NxT software
Quick reference card
1 vial of Attune™ Performance Tracking Beads
1 bottle of Attune™ Wash Solution
1 bottle of Attune™ 1X Shutdown Solution
Attune™ Focusing Fluid (10 L)
Format Tube(s)
Laser Features Blue Excitation Laser (488 nm), Yellow Laser (561 nm)
Product Line Attune
For Use With (Equipment) Attune™ NxT Acoustic Focusing Cytometer
Shipping Condition Room Temperature
Quantity 1 each

Frequently Asked Questions (FAQs)

I'd like to use the Flow Cytometry Panel Builder but will be purchasing antibodies from a different vendor than Thermo Fisher Scientific. Is this possible?

Step 2 allows for antibodies you have already purchased or are from a vendor outside Thermo Fisher Scientific. If you are undecided about which format you want for something, you can add the antigen name in the "Antibodies you need" section and then choose a placeholder fluorochrome in the following step. Both will help you keep track of channels that are reserved for reagents that you do not plan to buy from Thermo Fisher Scientific.
Can I export the spreadsheet from the Flow Cytometry Panel Builder for later use?

There are both PDF (printable) and CSV (spreadsheet) exports available in the summary step for you to take away for reference and ordering purposes.
In the Flow Cytometry Panel Builder tool, I can't find my flow cytometer in the dropdown. Can I build one?

Yes, you can define a new cytometer using the "Enter your cytometer manually" link below the cytometer dropdown menu.
I would like to use the Flow Cytometry Panel Builder but my flow cytometer does not use a standard configuration. How can I make changes to one in the dropdown?

Select a cytometer from the dropdown menu and then select the "Edit cytometer settings" link to modify the configuration as needed.
In the Flow Cytometry Panel Builder tool, what do the colorful dots indicate in the "Fluorochromes" step?

Each dot indicates a different fluorochrome that Thermo Fisher Scientific has available in each channel of the cytometer you have chosen.

How do I share my panel generated using the Flow Cytometry Panel Builder with my lab manager?

You may export the spreadsheet found on step 5 and share it.
In the Flow Cytometry Panel Builder, my marker isn't showing up under step 2 of antigen, but I see it in the catalog. How can I remedy this?

Select your target species first, and then type your antigen. A drop-down list will appear. Select the antigen from the list and click on "Confirm selections." If you have an antibody that is not on the list, in the "Antibodies you already have" section, you can enter a name and select "use this name anyway."

In the Flow Cytometry Panel Builder tool, can I go back a step without losing the work I did?

Yes, the previous work is saved. If you plan to leave the Panel Builder and come back later, be sure to select "Save" in the lower right corner to save your panel.

For Research Use Only. Not for use in diagnostic procedures.

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