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  6. Gibco™ B-27™ Supplement (50X), minus vitamin A

Gibco™ B-27™ Supplement (50X), minus vitamin A

Product Code. p-7075643
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11500446 10 mL 10mL
15440584 100 mL 100mL
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Product Code. 11500446 Supplier Gibco™ Supplier No. 12587010

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143.45 EUR valid until 2026-04-30
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Description

A variant of the original serum-free B-27 Supplement, designed for the proliferation of neural stem cells in the absence of vitamin A.

Gibco B-27 Supplement minus vitamin A is a customized, serum-free formulation based on the original, most cited neuronal cell culture supplement, designed specifically to support the proliferation and cultivation of neural stem cells (NSCs) and neural progenitor cells. By excluding vitamin A (also known as retinyl acetate or retinol), this variant prevents the differentiation-inducing effects of retinoic acid, making it ideal for maintaining NSCs and neural progenitors in their undifferentiated state.

  • Optimized for NSC proliferation—by removing vitamin A, this supplement is perfectly suited for the growth and expansion of embryonic and adult neural stem cells and neural precursors, without inducing differentiation
  • Versatile cultivation—supports the cultivation of neural progenitor cells and NSCs in both neurospheres in suspension and adherent monolayer cultures, providing flexibility in your experimental design. Ideal for cell culture where retinoic acid signaling must be controlled.
  • High quality—cGMP-manufactured to ensure the highest standards of quality and consistency. Each batch undergoes rigorous performance testing, including assessments of its ability to support the differentiation of human neural stem cells to neurons.
  • Comprehensive testing—every lot is tested for endotoxins, sterility, and mycoplasma to ensure the utmost safety and reliability
  • Proven performance—derived from the proprietary formulation of the original B-27 Supplement, trusted by researchers worldwide for its consistent and reliable results

Choose Gibco B-27 Supplement, minus vitamin A, for an optimized, high-quality solution that supports the proliferation of neural stem cells while preventing unwanted differentiation influenced by retinoic acid, ensuring consistent and reliable results for your research.

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store in freezer (-5 to -20°C) and protect from light.
Cell Type Neural Progenitor Cells
Classification Serum-free
Concentration 50 X
Culture Type Mammalian Cell Culture
Form Liquid
Product Type Cell Culture Supplement
Serum Level Serum-Free
Sterility Sterile
Product Line B-27
Quantity 10 mL
Shipping Condition Dry Ice
Source Serum-Free
Tested For Endotoxin, Performance, Sterility, Mycoplasma
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Frequently Asked Questions (FAQs)

Can B-27 supplement be filtered?

Yes, the protein content in this product is high enough so filtering through a low protein binding filter as a 50X or 1X in solution should not be a problem.

Can Neurobasal Medium alone or Neurobasal Medium supplemented with B-27 supplement be frozen to extend product shelf life?

No, it is not recommended to freeze media due to the potential of precipitates forming upon thaw. Inorganic salts and amino acids in the formulation may come out of solution when exposed to temperature fluctuations. These precipitates will not go back into solution easily once formed.
What supplements should I use with Neurobasal or Neurobasal-A medium?

Neurobasal and Neurobasal-A media (for postnatal and adult neurons) allow for long-term maintenance of neuronal cells without the need for an astrocyte feeder layer. These media should be supplemented with either serum or a serum-free supplement, plus 0.5mM L-glutamine. B-27 supplement is a serum-free supplement that comes as a 50X concentrate in a 10ml volume. This is enough supplement for 500ml of media. Fetal, postnatal, and adult neural cultures can be grown in the appropriate Neurobasal medium supplemented with B-27 supplement .

We also have two other supplements. One is called G-5 and is for growth and expression of glial cells (normal and tumor) of astrocytic phenotype. This comes in a 1ml size, at a 100X concentration. The other supplement is called N-2 and is for growth and expression of post-mitotic neurons and tumor cells of neuronal phenotype. This comes in a 5ml size, at a 100X concentration.

For more information on Neurobasal media, search "Neurobasal" from our website home page.

Do you offer B-27 supplements with and without insulin?

Yes, we do offer B-27 Supplement, minus insulin (Cat. Nos. A1895601 and A1895602). All our other B-27 supplements do contain insulin.
Can I thaw B-27 Supplements in a 37 degrees C water bath or incubator?

We do not recommend thawing at 37 degrees C. Best results are observed upon thawing at room temperature for approximately 2 hrs or overnight at 2-8 degrees C.
How do I thaw B-27 Supplements?

We recommend thawing it at 4 degrees C overnight or at room temperature for 2 hours. We don't recommend thawing in a water bath or incubator.
Can I re-freeze B-27 Supplements into smaller aliquots for future use?

Yes, we recommend that you aliquot when you first thaw it and thereafter subject the aliquot to no more than 2 additional freeze/thaw cycles.
What B-27 supplements do you offer?

We offer the following B-27 supplement products, which are developed for specific applications:

- Gibco B-27 Serum-free Supplement (Cat. No. 17504044): Complete formulation for the growth of long-term viability of neurons.
- Gibco B-27 Supplement minus AO (antioxidants) (Cat. No. 10889038): Five antioxidants are removed. The supplement is ideal in studies of oxidative damage, apoptosis, or applications in which free radical damage to neurons occurs.
- Gibco B-27 Supplement minus Insulin (Cat. No. A1895601): Insulin is removed. The supplement is ideal for studies of insulin secretion and insulin receptors.
- Gibco B-27 Supplement minus Vitamin A (Cat. No. 12587010): Vitamin A which induces neural differentiation is removed. The supplement is ideal for proliferation of stem cells.

Safety and Handling

compliance-icons
Product Identifier
  • 5-Methoxyindole
 Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H335-May cause respiratory irritation.
 Precautionary Statement
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
 Supplemental information
  • MIXTURE LIST-Contain: Indol-5-yl methyl ether

For Research Use Only. Not for use in diagnostic procedures.

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