Applied Biosystems™ cAMP-Screen™ Cyclic AMP Immunoassay System

• Utilizes highly sensitive chemiluminescent CSPD™ Substrate with Sapphire-II™ Enhancer that is triggered by an enzyme conjugate composed of cAMP bound to alkaline phosphatase (cAMP-AP)
• Ready-to-use chemiluminescent substrate/enhancer formulation generates sustained-glow light emission from 30 minutes after addition
• Mainly used in secondary screening and pre-clinical research, where sensitivity and no false positives are essential
• Highest sensitivity of any commercially available cAMP assay
• Hours of read time with little or no degradation of signal
• Simple protocol
• Useful for wide range of receptor activation studies

High Sensitivity and Hours of Steady Glow Time
This chemiluminescent assay is designed to provide the highest sensitivity of any commercially available cAMP assay. As few as 60 femtomoles of cAMP can be detected. The assay has a wide dynamic range, detecting from 0.06 to 6,000 picomoles of cAMP without the need for sample dilution or manipulations such as acetylation. This is especially helpful in cell-based assays when measuring Gs- or Gi-coupled agonist or antagonist stimulation and/or inhibition. Intra-assay precision for duplicate samples is typically 5% or less. Once the substrate/enhancer formulation reaches glow signal, the plate can be read for hours with little or no degradation of the signal. This is useful in screens where several plates are compared with each other. In addition, the assay exhibits exceptionally low cross-reactivity with other adenosine-containing or cyclic nucleotides.

For Receptor Activation Studies
The cAMP-Screen system is designed for quantitation of cellular cAMP for functional assays of receptor activation. The assay has been used with established cell lines for functional measurements with endogenous receptors, cell lines with exogenously expressed ligand receptors on the cell surface, primary cell cultures, and tissues in response to treatment with the appropriate ligands. In addition it has been used for receptor characterization, orphan receptor ligand identification, and the characterization of novel chimeric receptors. The assay can be used for high-throughput screens for compounds that stimulate or interfere with these signal transduction pathways.

Specifications

• Target: Cyclic AMP
• Assay Incubation Time: 1 hr.
• Assay Sensitivity: 60 femtomoles cAMP
• Detection Method: Chemiluminescent
• For use with Equipment: Microplate Reader
• Label/Dye: Alkaline Phosphatase
• Sample Type: Cell Lysates
• Sample Volume: 60μL
• Storage: Store at 2° to 8°C