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Thermo Scientific™ CaptureSelect™ CH1-XL GoPure™ Column

Product Code. 30275601 Shop All Thermo Scientific Products
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Product Code. 30275601 Supplier Thermo Scientific™ Supplier No. 6943462005

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Description

CaptureSelect CH1-XL GoPure Columns are ready-to-use columns designed for fast and easy purification of human IgG on standard chromatography systems with high purity and yield in a single step.

CaptureSelect CH1-XL GoPure Columns are ready-to-use columns designed for fast and easy purification of human IgG on standard chromatography systems with high purity and yield in a single step.

The pre-packed column contains CaptureSelect CH1-XL Affinity Matrix, which recognizes the CH1 domain of human IgG antibodies, enabling purification of all subclasses (1, 2, 3, and 4) of human IgG and Fab fragments, independent of the light-chain isotype and source material.

Features of this affinity matrix include:

  • Platform approach for purification of IgGs and their Fab fragments directly from complex source materials in a single step with high purity and yield
  • Elution possible at pH 4.0–4.5 with 50 mM acetate buffer
  • No co-purification of overproduced free light chains or light chain dimers
  • Specific for human antibodies; works in the presence of serum (bovine/horse)
  • Non-animal-derived

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created using a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics:

Matrix: agarose-based, epoxide-activated

Average particle size: 65 ± 10 μm

Ligand: CaptureSelect CH1-XL affinity ligand

Ligand coupling method: epoxide coupling

Binding capacity: >15 mg/mL Fab's depending on flow rate, column height, contact time

Elution conditions: 50 mM sodium acetate buffer, pH 4.0–4.5

Column dimensions: 1 mL column: 7 x 25 mm; 5 mL column: 16 x 25 mm

Max. recommended flow: 1 mL column: 6 mL/min; 5 mL column: 10 mL/min

Max. operation pressure: 2 bar

Formulation buffer: 20% (v/v) ethanol

Specifications

Max. Pressure 0.3 Mpa
Column Capacity 5 mL column
Quantity 5 mL
Particle Size 63 μm
Type Pre-packed Column
Diameter (Metric) Inner 16 mm
Content And Storage Store at 2°C to 8°C. Protect from direct daylight.
Product Line CaptureSelect
Matrix CH1-XL Affinity Matrix
Bed Volume 5 mL
Bed Height 25 mm
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For Research Use Only. Not for use in diagnostic procedures.

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