missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Protein Purification
  5. Protein Purification Reagents and Kits
  6. Thermo Scientific™ CaptureSelect™ LambdaXP GoPure™ Column

Thermo Scientific™ CaptureSelect™ LambdaXP GoPure™ Column

Product Code. 31722928 Shop All Thermo Scientific Products
Change view
Click to view available options
Quantity:
1 mL
5 mL
Unit Size:
1mL
5mL
2 product options available for selection
Product selection table with 2 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity Unit Size
31722928 1 mL 1mL
31722804 5 mL 5mL
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 2 options available.
2 options
This item is not returnable. View return policy
Product Code. 31722928 Supplier Thermo Scientific™ Supplier No. 6943752001
 Restricted Item
In Stock
Add to basket
Add to basket
This item is not returnable. View return policy

Description

CaptureSelect LambdaXP GoPure columns are ready-to-use columns designed for fast and easy purification of lambda monoclonal antibodies on standard chromatography systems with high purity and yield in a single step.

The pre-packed column contains CaptureSelect LambdaXP Affinity Matrix, which enables the purification of human lambda immunoglobulins (IgG, IgA, IgM, IgD, and IgE) and Fab fragments directly from complex source materials.

Features of this affinity matrix include:

  • Platform approach for purification of immunoglobulins and their Fab fragments with a Lambda light chain
  • Full coverage for all human Lambda subclasses
  • One-step purification from crude material with high purity and yield
  • No cross binding to the Fc region or kappa light chains
  • Excellent pH stability at 50–80 mM NaOH for limited exposure
  • Specific for human antibodies; works in the presence of serum (bovine/horse)
  • Non-animal-derived

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics

Matrix: agarose-based, epoxide-activated

Average particle size: 65 ± 10 μm

Ligand :CaptureSelect LambdaXP affinity ligand

Fab binding capacity: >20 mg IgG Lambda-Fab/mL resin, depending on residence time

IgG binding capacity: >35 mg IgG/mL resin, depending on residence time

Elution conditions: 20 mM citric acid or acetic acid, pH 3–4; 100 mM Tris, 1.5 M MgCl2, pH 6

Column dimensions: 7 x 25 mm (1 mL column); 16 x 25 mm (5 mL column)

Max. recommended flow: 5 mL/min. (1 mL column); 10 mL/min. (5 mL column)

Max. operation pressure: 2 bar

Formulation buffer: 20% (v/v) ethanol

Specifications

Bed Volume 1 mL
Capacity (Metric) Load capacity for 1 mL resin
Matrix LambdaXP Affinity Matrix
Content And Storage Store at 2°C to 8°C in dark/protect from light.
Diameter (Metric) 7 mm
Max. Operating Pressure 0.2 MPa
Particle Size 65 ±10 μm
Form Agarose Bead
Product Line CaptureSelect
Quantity 1 mL
Type Pre-packed Column
Bed Height 25 mm
Show More Show Less

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.