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CCF2-FA (free acid)

Product Code. 11514916
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Quantity:
100μg
1mg
Unit Size:
100µg
1mg
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Product Code. Quantity unitSize
11514916 100μg 100µg
10616883 1mg 1mg
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Product Code. 11514916 Supplier Invitrogen Supplier No. K1034

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Description

CCF2-FA (free acid)

CCF2-FA (Free Acid) is a fluorescence resonance energy transfer (FRET) substrate that consists of a cephalosporin core linking B7-hydroxycoumarin to fluorescein. This product is intended for use as a beta-lactamase substrate in a GeneBLAzer™ cell-based assay.

GeneBLAzer™ cell-based assays typically utilize the membrane-permeant ester forms (CCF2-AM and CCF4-AM) of the negatively charged fluorescent beta-lactamase substrates, CCF2 and CCF4. These lipophilic esters readily enter the cell, where cleavage by endogeneous cytoplasmic esterases rapidly converts them into their negatively charged forms, thereby trapping them in the cytosol.

CCF2-FA is essentially the CCF2 substrate without the esters found in the AM version. CCF2-FA is de-esterified and used in cell lysate applications, bypassing loading across the cell membrane and de-esterification steps. Cell lysates are the preferred method for applications using cells that contain a cell wall. CCF2-FA can also be used as a control to acquire the excitation and emission spectra for CCF2-AM and CCF4-AM.
TRUSTED_SUSTAINABILITY

Specifications

Cell Permeability Cell-Impermeant
Content And Storage Store in freezer (-5 to -30°C).
Detection Method Fluorescence
Form Powder
Product Line GeneBLAzer
Quantity 100μg
Substrate CCF2-FA
Substrate Properties Chemical Substrate
Substrate Type Beta-Lactamase Substrate
Shipping Condition Approved for shipment on Wet or Dry Ice

Safety and Handling

compliance-icons
Signal Word
  • Danger
 Hazard Category
  • Respiratory sensitiser Category 1
  • Skin sensitiser Category 1
 Hazard Statement
  • H317-May cause an allergic skin reaction.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
 Precautionary Statement
  • P302+P352-IF ON SKIN: Wash with plenty of water/.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P362-Take off contaminated clothing.
  • P272-Contaminated work clothing should not be allowed out of the workplace.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.

For Research Use Only. Not for use in diagnostic procedures.

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