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Gibco™ CD CHO Medium
Description
CD CHO medium is a protein-free, serum-free, chemically-defined medium optimized for the growth of Chinese hamster ovary (CHO) cells and expression of recombinant proteins in suspension culture. With a proven track record of quality for more than 10 years, CD CHO medium contains no proteins or peptide components of animal, plant, or synthetic origin, as well as no undefined lysates or hydrolysates.
When to Use CD CHO Medium
- Your GS CHO cells are recommended to be grown in CD CHO medium
- Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)
When to Consider Another Gibco™ Medium
Consider CD FortiCHO™ medium when:
- The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
- Maximum batch culture cell densities and protein titers are needed
- You have time to adapt cells into a new medium in an effort to maximize titers
- Cell health at harvest is a priority for downstream processing
Consider CD OptiCHO™ medium when:
- You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
- Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
- Your CHO cell line is 'finicky,' i.e. hard to grow or adapt
Consider CD DG44 medium when:
- You use parental DG44 or DXB11 CHO cells (dhfr-)
Dry Media Formulations
Gibco™ liquid media are available in an AGT™ (Advanced Granulation Technology) dry media format that is designed to provide increased consistency and productivity across all stages of production from development to commercial manufacturing. AGT™ media are manufactured animal origin-free with the same complete, serum-free, protein-free, chemically-defined formulation and performance as their liquid media counterparts.
Design Your Own Media Specific to Your Needs
Whether it's a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs.
Specifications
Specifications
| Cell Line | CHO |
| Classification | Animal Origin-free, Chemically-defined, Protein-free, Serum-free |
| Concentration | 1 X |
| Form | Liquid |
| Product Type | CD CHO Medium |
| Serum Level | Serum-free |
| With Additives | Sodium Pyruvate, Sodium Bicarbonate |
| Without Additives | No Glutamine, No Phenol Red, No Thymidine, No Hypoxanthine |
| Product Line | Gibco |
| Quantity | 0.5 L |
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Frequently Asked Questions (FAQs)
Thermo Fisher Scientific offers multiple serum free media (SFM) and chemically defined (CD) media options for CHO cultures. For adherent cultures, CD CHO (10743-011) and CD OptiCHO medium (12681-011) are robust media; both are protein free and animal origin free (AOF). For adherent cultures, CD CHO-A (097-0182DJ) is a widely used protein free and animal origin free medium. There are other formulations available to suit other research needs; contact Technical Support for further information.
Cells can be adapted by Sequential or Direct Adaptation. Suggested protocols for each are below, and you can also find more information by searching "Adaptation of Cell Cultures to a Serum-Free Medium" from our website home page.
SEQUENTIAL ADAPTATION
1) Subculture the cells growing in serum-supplemented medium into a 25%:75% mixture of SFM and serum supplemented medium.
2) When the cell density is 5 x 10E5 cells/ml, subculture the cells into a 50%:50% mixture of SFM and serum supplemented medium at a cell density 2.5 x 10E5 to 3 x 10E5 cells/ml.
3) Continue to subculture after the cell density 5 x 10E5 cells/ml in gradually increasing proportions of SFM until the serum is ~0.1% with about 85% cell viability.
4) Subculture the cells into SFM with an innoculum of 2.5 x 10E5 to 3 x 10E5 cells/ml.
5) When the cell density is 1 x 10E6 to 3 x 10E6 cells/ml (4 to 6 days post planting) subculture the cells again.
6) Stock cultures of SFM adapted cells should be subcultured in SFM every 3 to 5 days when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml with 90% viability.
DIRECT ADAPTATION
Some cells can be directly adapted from serum-containing medium to SFM. For direct adaptation, the cell innoculum should be 1.5 x 10E5 to 3 x 10E5 cells/ml.
Cells should be subcultured when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml. Cells are fully adapted to SFM when the cell density is 2 x 10E6 to 4 x 10E6 cells/ml after 4 to 7 days in culture.
Stock cultures of cells adapted to SFM should be subcultured in SFM every 3 to 5 days when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml with 90% viability.
Some cells, such as insect cells, are sensitive to changes in their medium. By sequentially adapting cells, the medium is changed with minimal effects on cell growth.
While the full formulation for 1X CD CHO Medium is proprietary information that cannot be disclosed, we can confirm that the medium contains a surfactant and that no additional surfactant would need to be added to the medium to support your suspension CHO cells in shake flask culture.
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