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IBA Lifesciences CD3/CD62L/CD45RA Fab-TACS™ Magnetic Microbead Starter Kit

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Product Code. 17247760

Brand: IBA Lifesciences 68000116

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Beschreibung

Label-free isolation of naive or central memory T cells from PBMCs

  • Fab-TACS™ - Traceless Affinity Cell Selection - is a positive cell isolation approach based on our Strep-tag™ technology
  • CD3/CD62L/CD45RA Fab-TACS™ Magnetic Microbead Starter Kit allows label-free isolation of CD3+ central memory (CD3+CD62L+CD45RA-) or naive (CD3+CD62L+CD45RA+) T cells from peripheral blood mononuclear cells (PBMCs)
  • Alternatively, CD3/CD62L/CD45RO Fab-TACS™ Magnetic Microbead Starter Kit is suitable for the isolation of these cells
  • Low affinity, Fab fragments fused to a Twin-Strep-tag™ (Fab-Streps) are incubated with Strep-Tactin™ Magnetic Microbeads to form a complex that stably binds to the cell surface
  • After specific labeling, target cells are bound to a magnet and non-target cells efficiently washed away
  • In a final step, the addition of biotin causes the release of the isolated cells from magnetic microbeads. The Fab-Streps self-dissociate from the cell surface due to their low affinity
  • After removing biotin by washing, the isolated cells are ready for further positive selection or depletion steps or can directly be used for further downstream applications

Spezifikation

Includes Strep-Tactin™ Magnetic Microbeads, CD3/CD62L/CD45RA Fab-Strep (human), 10 x Buffer CI, Biotin stock solution
Target Species Human
For Use With (Application) Label-Free Isolation of Naive or Central Memory T Cells From PBMCs

For Research Use Only.

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