BB700 Mouse Anti-Human CD56 Clone NCAM16.2 (NCAM16) (RUO)

Description

• The NCAM16.2 monoclonal antibody specifically binds to human CD56.
• It recognizes an extracellular immunoglobulin-like domain common to 120, 140, and 180 kDa forms of CD56, also known as the neural cell adhesion molecule (NCAM), NKH1 or MSK39.
• The CD56 antigen is expressed on approximately 10% to 25% of peripheral blood lymphocytes.
• It is present on essentially all resting and activated CD16+ natural killer (NK) lymphocytes and approximately 5% of CD3+ peripheral blood lymphocytes. CD3+ CD56+ T lymphocytes comprise a unique subset of cytotoxic T lymphocytes that mediates non-major histocompatibility complex (MHC)-restricted cytotoxicity.
• CD56 antigen density on NK lymphocytes increases upon cellular activation.
• The CD56 antigen is involved in neuronal homotypic cell adhesion and cell differentiation during embryogenesis.
• CD16+ CD56+ NK cells demonstrate reciprocal transfer of an activation state with dendritic cells.
• The antibody was conjugated to BD Horizon BB700, which is part of the BD Horizon Brilliant™ Blue family of dyes.
• It is a polymer-based tandem dye developed exclusively by BD Biosciences.
• With an excitation max of 485 nm and an emission max of 693 nm, BD Horizon BB700 can be excited by the 488 nm laser and detected in a standard PerCP-Cy™5.5 set (eg, 695/40-nm filter).
• This dye provides a much brighter alternative to PerCP-Cy5.5 with less cross laser excitation off the 405 nm and 355 nm lasers.