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IBA Lifesciences™ CD8/CD62L/CD45RO Fab-TACS™ Magnetic Microbead Starter Kit, Human

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Quantity:
1 Kit
Unit Size:
1 set
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Product Code. 17500655

Brand: IBA Lifesciences™ 68000110

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Description

CD8/CD62L/CD45RO Magnetic Microbead Starter Kit for the label-free isolation of human central memory T cells from PBMCs.

Fab-TACS™ Traceless Affinity Cell Selection is a positive cell isolation approach based on our Strep-tag™ technology. CD8/CD62L/CD45RO Fab-TACS™ Magnetic Microbead Starter Kit allows label-free isolation of human CD8+ central memory T cells (CD8+CD62L+CD45RO+) from peripheral blood mononuclear cells (PBMCs). Alternatively, CD8/CD62L/CD45RA Fab-TACS™ Magnetic Microbead Starter Kit is suitable for the isolation of these cells.

Low affinity, Fab fragments fused to a Twin-Strep-tag™ (Fab-Streps) are incubated with Strep-Tactin™ Magnetic Microbeads to form a complex that stably binds to the cell surface. After specific labeling, target cells are bound to a magnet and non-target cells are efficiently washed away. In the final step, the addition of biotin causes the release of the isolated cells from magnetic microbeads. The Fab-Streps self-dissociate from the cell surface due to their low affinity. After removing biotin by washing, the isolated cells are ready for further positive selection or depletion steps or can directly be used for further downstream applications.

  • High purity of >90%
  • Scalable reagents
  • Label-free cells due to low affinity Fab fragments
  • Non-activated cells

Specifications

Includes Strep-Tactin™ Magnetic Microbeads, CD8 Fab-Strep, CD62L Fab-Strep, and CD45RO Fab-Strep (Human), 10X Buffer CI, 100 mM Biotin Stock Solution
Product Type Cell Isolation Kit
Cell Type Human CD8/CD62L/CD45RO+ Cells
Target Species Human
For Use With (Application) Magnetic Cell Isolation
Buffer 10X Buffer CI
Sufficient For 5 x 107 Total Cells
Quantity 1 Kit
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