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Thermo Scientific™ CellSensor™ NFAT CHO-K1 DA Assay Kit

Product Code. 10338674
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Product Code. 10338674 Supplier Thermo Scientific™ Supplier No. K1447

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Description

The CellSensor™ NFAT CHO-K1 DA (Division Arrested) cells contain a beta-lactamase (bla) reporter gene under control of a Nuclear Factor of Activated T Cells (NFAT) response element stably integrated into CHO-K1 cells.

The CellSensor™ NFAT CHO-K1 DA (Division Arrested) cells contain a beta-lactamase (bla) reporter gene under control of a Nuclear Factor of Activated T Cells (NFAT) response element stably integrated into CHO-K1 cells. Fluorescence activated cell sorting (FACS) was used to isolate clones responsive to stimulation of the NFAT pathway by PMA (Phorbol 12-Myristate 13-Acetate) and Thapsagargin (Thaps). The cell line was validated for DMSO tolerance, cell number, stimulation time, substrate loading conditions, and EC50 concentration of Thaps at a fixed concentration of PMA. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction

This product can serve as a negative control in screening assays performed with specific G-protein coupled receptor (GPCR) DA cells that were made from this same background. These cells can also be used in other assays where a sensitive readout to intracellular changes in calcium is needed.

TRUSTED_SUSTAINABILITY

Specifications

Cell State Division-Arrested Cells
Content And Storage Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol).

Includes:
• CellSensor™ NFAT CHO-K1 DA cells
• LiveBLAzer™-FRET B/G Loading Kit, 70 μg
• Solution D
• CellSensor™ NFAT CHO-K1 Assay Protocol
• LiveBLAzer™-FRET B/G Loading Protocol
• Certificate of Analysis

The CellSensor™ NFAT CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Description CellSensor™ NFAT CHO-K1 DA Assay Kit, 1Vial, ∽ 2,400,000 cells/vial Quantity of Cells, GeneBLAzer™ Druggable Target, Fluorescent Detection Method, Adherent Growth Properties, Liquid Nitrogen Storage
Detection Method Fluorescent
For Use With (Application) Pharma and biopharma, drug discovery and development, target and lead identification and validation, pathway biology, cellular pathway analysis assays, cellsensor™ cellular pathway assays
Includes beta-lactamase (bla) reporter gene under control of a nuclear factor of activated T cells (NFAT) response element stably integrated into CHO-K1 cells
Product Line CellSensor
Quantity 1 Plate
Type Division-Arrested Cells Assay Kit
System Type GeneBLAzer™
Druggable Target Signaling Pathway
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For Research Use Only. Not for use in diagnostic procedures.

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