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Thermo Scientific™ CellSensor™ NFAT HEK 293T DA Assay Kit

Product Code. 10616645
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Product Code. 10616645 Supplier Thermo Scientific™ Supplier No. K1455

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Description

The CellSensor™ NFAT HEK 293T DA (Division Arrested) cells contain a beta-lactamase reporter gene under the control of the Nuclear Factor of Activated T Cells (NFAT) response element stably integrated into HEK 293T cells.

The CellSensor™ NFAT HEK 293T DA (Division Arrested) cells contain a beta-lactamase reporter gene under the control of the Nuclear Factor of Activated T Cells (NFAT) response element stably integrated into HEK 293T cells. Fluorescence activated cell sorting (FACS) was used to isolate clones responsive to stimulation of the NFAT pathway by PMA (Phorbol 12-Myristate 13-Acetate) and Thapsagargin (Thaps). The cell line was validated for DMSO tolerance, cell number, stimulation time, substrate loading conditions, and EC50 concentration of Thaps at a fixed concentration of PMA. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

This product can serve as a negative control in screening assays performed with specific G-protein coupled receptor (GPCR) DA cells that were made from this same background or these cells can be used in other assays where a sensitive readout to intracellular changes in calcium are needed.

TRUSTED_SUSTAINABILITY

Spécification

Cell State Division-Arrested Cells
Content And Storage Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol).

Includes:
• CellSensor™ NFAT HEK293T DA cells
• LiveBLAzer™-FRET B/G Loading Kit, 70 μg
• Solution D
• CellSensor™ HEK293T CHO-K1 Assay Protocol
• LiveBLAzer™-FRET B/G Loading Protocol
• Certificate of Analysis

The CellSensor™ NFAT HEK 293T DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application) GPCRs, Signaling Pathway
Includes CellSensor™ NFAT HEK293T DA cells, LiveBLAzer™-FRET B/G Loading Kit 70 μg, Solution D, CellSensor™ HEK293T CHO-K1 Assay Protocol, LiveBLAzer™-FRET B/G Loading Protocol, Certificate of Analysis
Product Line CellSensor
Quantity 1 plate
Type Division-Arrested Cells Assay Kit
System Type CellSensor™
Druggable Target Signaling Pathway

For Research Use Only. Not for use in diagnostic procedures.

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