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CLA IdentiFiler™ Plus PCR Amplification Kit

Product Code. 31722768 Shop All Applied Biosystems Products
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Code produit. 31722768 Fournisseur ABI Applied Biosystems Code fournisseur A47624

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Description

The CLA (Cell Line Authentication) IdentiFiler Plus PCR Amplification Kit simplifies the amplification step during authentication of cell lines.

The CLA (Cell Line Authentication) IdentiFiler Plus PCR Amplification Kit includes the same primers and allelic ladder as the widely used AmpFLSTR Identifiler Plus kit and harnesses next-generation PCR amplification technology to help provide enhanced sensitivity, a cleaner baseline, improved performance with mixtures, and the ability to overcome high levels of PCR inhibition.

Features of the CLA IdentiFiler Plus PCR Amplification Kit include:
• 5-dye, 16-locus STR kit compatible with all cell line databasing loci standards
• Improved version of legacy AmpFLSTR Identifiler Plus kit
• Compatible with SeqStudio, 3730/xL, and 3500/xL genetic analyzers

Cell line authentication using short tandem repeat (STR) analysis kits can be used both for human sample authentication (HSA) and mixed sample analysis (MSA). In HSA, there is a need to verify the DNA profile of the sample to check for sample cross-contamination or sample mix-up, or to perform a quality check. For MSA there is a need to deconvolute the presence of multiple contributors in a sample (chimerism) through identification of multiple DNA genotypes in the sample.

TRUSTED_SUSTAINABILITY

Spécification

Content And Storage

• 1 AmpFLSTR Identifiler Plus PCR Amplification Kit:

  • AmpFlSTR Identifiler Plus Master Mix - 1 tube at 0.5 mL
  • AmpFlSTR Control DNA 9947A - 1 tube at 0.3 mL
  • AmpFlSTR Identifiler Plus Primer Set - 1 tube at 0.25 mL
  • AmpFlSTR Identifiler Plus Allelic Ladder - 1 tube at 0.025 mL

• Store at -5°C to -30°C
Detection Method Primer-probe
Format Tube
GC-Rich PCR Performance High
Reaction Speed Standard
Technique SNP Genotyping (Fragment Analysis-Based), STR (Short Tandem Repeat) Analysis
Genotyping Target SNPs (Unknown or Numerous)
Product Type Plus PCR Amplification Kit
Quantity 50 reactions
Shipping Condition Wet Ice
Species Human
No. of Reactions 50 Reactions
Volume 0.5 mL
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For Cell Line Authentication Use excluding, Forensic, Paternity, Diagnostic, and Therapeutic applications.

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