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  6. Invitrogen™ Click-iT™ TUNEL Alexa Fluor Imaging Assays for Microscopy & HCS 

Invitrogen™ Click-iT™ TUNEL Alexa Fluor Imaging Assays for Microscopy & HCS
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Product Code. 10338924
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Color:
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Label or Dye:
Alexa Fluor 488, Hoechst 33342
Alexa Fluor 594, Hoechst 33342
Alexa Fluor 647, Hoechst 33342
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Each
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Product Code. Color Label or Dye unitSize
10338924 Far-Red Alexa Fluor 647, Hoechst 33342 Each
10024014 Red Alexa Fluor 594, Hoechst 33342 Each
10798585 Green Alexa Fluor 488, Hoechst 33342 Each
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Product Code. 10338924 Supplier Invitrogen™ Supplier No. C10247
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Descrizione

Includes

One kit containing sufficient reagents to perform 1 x 96 tests or 50 coverslips.

Screen apoptotic cells with Click-iT TUNEL Assay kits, which offer easy Alexa Fluor dye incorporation and can be multiplexed with GFP and RFP.

Screen apoptotic cells easily and efficiently in just two hours with the Click-iT TUNEL Alexa Fluor 488, 594, and 647 imaging assays. Intended for use with microscopy and high-content screening (HCS), these TUNEL assay kits use a copper-catalyzed reaction to detect incorporated alkyne-modified dUTPs on terminal DNA ends of fragmented DNA. Compared with assays using other modified nucleotides, these apoptosis detection kits are fast (complete within two hours) and can detect a higher percentage of apoptotic cells under identical conditions. Click-iT TUNEL assays also allow multiplexing with surface and intracellular biomarker detection methods, including those that measure fluorescent signal from GFP or RFP.

Apoptosis is marked by defined cellular processes, including cell rounding, blebbing, and DNA fragmentation. The TUNEL assay is the most widely used analytical method to detect fragmented DNA in apoptotic cells in tissue samples, beginning with the incorporation of modified dUTPs at the 3’-OH ends of fragmented DNA. The dUTPs often include a fluorophore molecule. Due to the size of the fluorophore, modified dUTPs can display lower than expected incorporation rates, negatively affecting the sensitivity of the TUNEL assay. Quite often, fluorophores used in currently available TUNEL assay kits exhibit photobleaching and fluorescent spectral overlap issues, both of which reduce the sensitivity of and ability to multiplex these assays.

The Click-iT Plus TUNEL assay was developed to address these issues and is based on a copper (I) catalyzed (i.e., click) reaction between an azide and alkyne. The small size of the Alexa Fluor azide, which has a molecular weight of about 1 kDa, enables easier incorporation of the Alexa Fluor 488, 594, or 647 dye into complex samples, as compared to larger (MW ˜100,000 Da) antibodies. This permits mild fixation or permeabilization and faster assay turnaround time (about two hours) and enables the detection of a higher percentage of apoptotic cells under identical conditions. Because of its gentle reaction conditions, the Click-iT Plus TUNEL assay can also be multiplexed with fluorescent proteins or dyes.

The Click-iT TUNEL Alexa Fluor imaging assays contain all components needed to accurately and reliably detect apoptosis in adherent cells grown on coverslips or 96-well microplates, and they also include DNase I to generate strand breaks for the positive control.

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Shipping Condition: Dry ice

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Specifica

Description Click-iT TUNEL Alexa Fluor™ 647 Imaging Assay
Quantity 1 kit
Format 96-well Plate
Product Type Imaging Assay
Content And Storage Store at ≤-20°C and protected from light.
Excitation/Emission 650/665
No. of Reactions 1 X 96 tests or 50 coverslips
Color Far-Red
Shipping Condition Dry Ice
Product Line Click-iT
Detection Method Fluorescence
For Use With (Equipment) Fluorescence Microscope, High Content Instrument
Label or Dye Alexa Fluor 647, Hoechst 33342
Label Type Alexa Fluor™ Dyes, Classic Dyes
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