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STEMCELL Technologies DNase I, ACF

Product Code. 17149281
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Quantity:
100,000 U
25,000 U
500,000 U
Unit Size:
1 set
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Product Code. 17149281

Brand: STEMCELL Technologies 07473

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This item is not returnable. View return policy

Description

Animal component-free DNase I for digestion of DNA

  • Deoxyribonuclease I (DNase I), Animal Component-Free (ACF) is obtained from cultures free of animal-derived materials and purified by chromatography
  • DNase I is an endonuclease consisting of a single glycosylated polypeptide chain with two disulfide bonds
  • DNase is often included in tissue dissociation protocols to digest DNA that has leaked into the dissociation medium as a result of cell damage
  • DNase I, ACF preferentially cleaves phosphodiester linkages adjacent to pyrimidine nucleotides in both single- and double-stranded DNA, yielding polynucleotides with 5'-phosphate and 3'-hydroxyl groups (Bernardi et al.) DNase I has been used for the digestion of human tissues such as microglia (Klegeris & McGeer), cartilage (Dunham & Koch), colon (Fukushima & Fiocchi), epithelium l (Fukushima & Fiocchi), liver (Vatakis et al.), lung (Fujino et al.), neural (Fuja et al.), and stem cells (Kusuma et al.)

Specifications

For Use With (Application) Digestion of DNA
Quantity 25,000 U
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