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Description
Only template DNA and PCR primers need to be added by the user. The modified DNA polymerase in this kit incorporates a non-specific DNA-binding domain that lends physical stability to the polymerase-DNA complex. ROX passive reference dye is also supplied with the kit in a separate tube for instruments that require ROX normalization.
- Strong specificity: DyNAmo HS SYBR Green master mix minimizes the formation of unwanted primer-dimers and extension of non-specifically bound primers during reaction setup
- Highly sensitive detection: Optimized master mix allows reproducible detection of low-copy number templates
- Broad dynamic range: DyNAmo HS SYBR Green qPCR Kit detects as few as one copy of the target gene and achieves a dynamic range of up to eight orders of magnitude
- Convenient to use: 2X master mix, one tube only; ROX passive reference dye provided in a separate vial
Specifications
Specifications
| Concentration | 2X (qPCR Mix), 50X (Dye) |
| Content And Storage | Contains: • 25 x1 mL DyNAmo HS SYBR Green qPCR Mix (2X) • 1 x 1.25 mL 50 x ROX dye, 25μM Store at -20°C |
| Detection Method | SYBR |
| Format | Tube |
| GC-Rich PCR Performance | High |
| PCR Method | qPCR |
| Polymerase | Tbr DNA polymerase |
| Reaction Speed | Standard |
| For Use With (Equipment) | 7000 System, 7300 System, 7500 Fast System, 7500 System, 7700 System, 7900HT Fast System, 7900HT System, BioRad CFX96™, QuantStudio™, ViiA™ 7 System, Bio-Rad CFX384 Touch™, Roche LightCycler 480, 5700 Sequence Detection System, StepOnePlus™ System, Eppendorf MasterCycler RealPlex, Corbett RotorGene, StepOne™ System, Stratagene Mx4000, Stratagene Mx3005P, Stratagene Mx3000P, MJ Opticon, MJ Chromo4, Cepheid SmartCycler |
| Kit Type | DyNAmo HS SYBR Green qPCR Kit |
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For Research Use Only. Not for use in diagnostic procedures.
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