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Thermo Scientific™ E. coli DNA Ligase
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Quantity:
400 U
Unit Size:
Each
Description
Thermo Scientific™ E. coli DNA Ligase is an NAD-dependent DNA ligase. It catalizes formation of phosphodiester bonds between 5`-phospate and 3`-hydroxyl termini in double-stranded DNA. The enzyme is not active on blunt-ended DNA.
Features
- Activity in wide temperature range (4°C to 37°C)
- High specificity to cohesive DNA ends
Applications
- Nick ligation in double-stranded DNA
- cDNA synthesis
- Cloning
Specifications
Specifications
| Concentration | 8 U/μL |
| For Use With (Application) | Nick ligation in double-stranded DNA, cDNA synthesis, Cloning, DNA ligation |
| Content And Storage | 8 U/μL E.Coli DNA Ligase, 50 μL Store at -20°C. |
| Enzyme | DNA Ligase |
| Sensitive to Heat Inactivation | Yes |
| Quantity | 400 U |
| Product Type | NAD-dependent DNA Ligase |
| Research Category | Traditional Cloning |
| Inactivation Temperature | 65°C |
| Form | Liquid |
For Research Use Only. Not for use in diagnostic procedures.
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