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Thermo Scientific™ Exonuclease I (20 U/μL)

Product Code. 10548930 Shop All Thermo Scientific Products
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To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27969” to get your promotional price
Code produit 10548930 Fournisseur Thermo Scientific™ Code fournisseur EN0581

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80.67 EUR valid until 2026-06-26
BEST PRICE on Promo! Use promo code "27969" to get your promotional price.
Les retours ne sont pas autorisés pour ce produit. Afficher la politique du retour.
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27969” to get your promotional price

Description

Degrade single-stranded DNA in a 3'→5' direction with Exonuclease I (ExoI), leaving 5'-terminal dinucleotides intact.

Exonuclease I (ExoI) degrades single-stranded DNA in a 3'≥5' direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaves 5'-terminal dinucleotides intact.

It does not cleave DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups.

Highlights

  • Active in PCR buffers

Applications

  • Primer removal from PCR mixtures:
    • prior to PCR product sequencing
      • li>for one-tube "megaprimer" PCR mutagenesis
  • Removal of single-stranded DNA containing a 3'-hydroxyl terminus from nucleic acid mixtures
  • Assay for the presence of single-stranded DNA with a 3'-hydroxyl terminus

Note

The enzyme is not suitable for removing 3'-overhangs of dsDNA.

TRUSTED_SUSTAINABILITY

Spécification

Concentration 20 U/μL
Enzyme Exonuclease
Quantity 4,000 units
Product Type Exonuclease I

Sécurité et traitement

compliance-icons
Identifiant produit
  • Exonuclease I
 Mention d'avertissement
  • Warning
 Catégorie de danger
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
 Mention de danger
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
 Conseils de prudence
  • P264-Wash hands thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P332+P313-If skin irritation occurs: Get medical advice/attention.
  • P337+P313-If eye irritation persists: Get medical advice/attention.
  • P362+P364-Take off contaminated clothing and wash it before reuse.
 Informations supplémentaires
  • MIXTURE LIST-Contain: Potassium hydroxide

For Research Use Only. Not for use in diagnostic procedures.

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