F(ab')2-Goat anti-Human Kappa Light Chain Secondary Antibody, PE, Invitrogen™

Description

This antibody has been cross-absorbed with pooled human myeloma proteins with lambda light chains. Purified IgG was prepared from whole serum by affinity chromatography, and F(ab)2 fragments were prepared by pepsin digestion. For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Goat F(ab')2 anti Human Kappa Light Chain antibody recognizes human kappa light chains. This antibody has been cross absorbed with pooled human myeloma proteins with lambda light chains.

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.