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Description
Thermo Scientific GeneJET Plasmid Midiprep Kit, sufficient for 25 preps, is designed for large scale isolation of high quality plasmid DNA from recombinant E. coli cultures. The kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200 μg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.
Highlights
- Efficient—high yields up to 200 μg of high quality plasmid DNA from 50 mL of bacterial culture
- High concentration of DNA purified— >400 ng/μL
- Convenient—spin column format, centrifuge or vacuum protocols
- Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns
Applications
Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
- FastDigest restriction enzyme digestion
- Automated fluorescent sequencing
- PCR
- Cloning
- Transformation
- In vitro transcription
- Transfection of robust cell lines
Specifications
Specifications
| Concentration | 400 ng/μL |
| Column Type | Spin Column |
| Format | Spin Column |
| Isolation Technology | Silica Column |
| Scale | Midi |
| Sample Type | Bacteria |
| For Use With (Application) | Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| Prep Scale | 100-200 μg (Medium-Scale) Plasmid DNA |
| Product Line | GeneJET |
| Show More |
Safety and Handling
- GeneJET Plasmid Midiprep Kit
- Warning
- Acute toxicity Category 4
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor / soap
- P302+P352-IF ON SKIN: Wash with plenty of water/.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P330-Rinse mouth.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P362+P364-Take off contaminated clothing and wash it before reuse.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contain: guanidine hydrochloride, sodium lauryl sulphate, sodium hydroxide, acetic acid,triton X-100
For Research Use Only. Not for use in diagnostic procedures.
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