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Thermo Scientific™ LguI (SapI) (5 U/μL)
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Description
Lambda DNA digested with LguI (SapI), 0.7% agarose, 10 cleavage sites
conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
5'..G C T C T T C (N)1▵...3'
3'..C G A G A A G (N)4▵...5'
Conditions for 100% Activity:
- 1X Buffer Tango™: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
- Incubate at 37°C
Storage Buffer:
- LguI is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol
Methylation Effects:
- Dam: never overlaps —no effect
- Dcm: never overlaps —no effect
- CpG: may overlap —no effect
- EcoKI: never overlaps —no effect
- EcoBI: may overlap —effect not determined
Digestion of Agarose-embedded DNA:
- Minimum 5U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours
Note:
Low salt, high glycerol (>5%) concentrations, pH >8.0 or a large excess of enzyme may result in star activity.
Specifications
Specifications
| Concentration | 5 U/μL |
| Methylation Sensitivity | Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive |
| Enzyme | LguI (SapI) |
| Compatible Buffer | 10X Buffer Tango |
| Sensitive to Heat Inactivation | Yes |
| Optimal Reaction Temperature | 37°C |
| Type IIS RE | Yes |
| Quantity | 100 units |
| Product Type | Restriction Enzyme |
| Research Category | Scarless Cloning |
For Research Use Only. Not for use in diagnostic procedures.
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