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Thermo Scientific™ PvuII, HC (50 U/μL)
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Thermo Scientific™ PvuII is available as a FastDigest enzyme which is used for rapid DNA digestion, more than 90% of the digested DNA fragments can be ligated and more than 95% of these sites can be recut at a 5'-termini concentration of 0.15 μM after a 10-fold overdigestion (5U/μg DNA x 2 hours) with PvuII.
5'...C A G▵C T G...3'
3'...G T C▵G A C...5'
Conditions for 100% Activity
- 1X Buffer G:10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 50mM NaCl and 0.1mg/mL BSA
- Incubate at 37°C
Storage Buffer
- PvuII is supplied in:10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol
Ligation and Recleavage
- After 10-fold overdigestion with PvuII, more than 90% of the DNA fragments can be ligated and more than 95% of these can be recut
Methylation Effects
- Dam: never overlaps —no effect
- Dcm: never overlaps —no effect
- CpG: never overlaps —no effect
- EcoKI: never overlaps —no effect
- EcoBI: may overlap —effect not determined
Digestion of Agarose-embedded DNA
- Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours
Note:
Greater than 15-fold overdigestion with PvuII may result in star activity.
Specifications
Specifications
| Concentration | 50 U/μL |
| Methylation Sensitivity | Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive |
| Enzyme | Pvu II |
| Compatible Buffer | 10X Buffer G |
| Sensitive to Heat Inactivation | No |
| Optimal Reaction Temperature | 37°C |
| Type IIS RE | No |
| Quantity | 12,500 units |
| Product Type | Restriction Enzyme |
| Research Category | Traditional Cloning |
For Research Use Only. Not for use in diagnostic procedures.
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