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Thermo Scientific™ RNase I (10 U/μL)

Product Code. 10568930 Shop All Thermo Scientific Products
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Product Code. 10568930 Supplier Thermo Scientific™ Supplier No. EN0601

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86.00 EUR valid until 2026-06-26
BEST PRICE on Promo! Use promo code "27969" to get your promotional price.
This item is not returnable. View return policy
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27969” to get your promotional price

Beskrivning

Ribonuclease I (RNase I) is an endoribonuclease that hydrolyzes single-stranded RNA to nucleoside 3'-monophosphates.

Ribonuclease I (RNase I), an endoribonuclease, preferentially hydrolyzes single-stranded RNA to nucleoside 3'-monophosphates via nucleoside 2', 3'-cyclic monophosphate intermediates.

The enzyme does not require any metal ions for activity.

Highlights

  • Stable and active under a wide variety of reaction conditions
  • Can be heat inactivated in 30 minutes at 100°C
TRUSTED_SUSTAINABILITY

Specifikationer

Concentration 10 U/μL
Enzyme RNase
Quantity 1,000 units
Product Type RNase I

Frequently Asked Questions (FAQs)

What are your recommendations for removal of RNA from DNA solutions using Thermo Scientific RNase I?

1. Add 15-20 u of RNase I per 1 µg of RNA. RNase I is ≥ 90% active within pH range 7.0-8.8 at salt concentration 100-200 mM. Incubation with RNase I can be performed simultaneously with the digestion of DNA by restriction endonucleases.

2. Incubate at 37 degrees C for 30 minutes.

3. Purify DNA by spin column or phenol/chloroform extraction.

For Research Use Only. Not for use in diagnostic procedures.

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