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Thermo Scientific™ GeneBLAzer™ CALCR -CRE-bla FreeStyle™ 293F Cells

Product Code. 10053783
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10053783 2 x 106 Cells Each
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Product Code. 10053783 Supplier Thermo Scientific™ Supplier No. K1480

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Description

The GeneBLAzer™ CALCR-CRE-bla Freestyle293 cells contain the CALCR receptor isoform (CTR2/CT(a)/CTRI1-), Accession No. NM_001742.2, stably integrated into the CellSensor™ CRE-bla Freestyle293 cell line.

The GeneBLAzer™ CALCR-CRE-bla Freestyle293 cells contain the CALCR receptor isoform (CTR2/CT(a)/CTRI1-), Accession No. NM_001742.2, stably integrated into the CellSensor™ CRE-bla Freestyle293 cell line. The CellSensor™ CRE-bla Freestyle293 cells contain a beta-lactamase (bla) reporter gene under control of the cAMP (CRE) response element.

CALCR-CRE-bla Freestyle293 cells have been tested for variable assay conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time and functionally validated for Z' and EC50 concentrations of calcitonin. Additional testing data using alternate stimuli are also provided. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section.

TRUSTED_SUSTAINABILITY

Specifications

Accession Number NM_001742.2
Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.

Includes:
• GeneBLAzer™ CALCR-CRE-bla Freestyle™ 293F cells
• GeneBLAzer™ CALCR-CRE-bla Freestyle™ 293F Assay Protocol
• Certificate of Analysis

The GeneBLAzer™ CALCR-CRE-bla Freestyle™ 293F cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Description GeneBLAzer™ CALCR -CRE-bla FreeStyle™ 293F Cells, 2 x 106 Cells, ∽2,000,000 cells/vial at 2,000,000 cells/mL Quantity of Cells, FreeStyle™ 293-F Cell Line, Fluorescent Detection Method, GPCRs Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage
Detection Method Fluorescence
For Use With (Application) Pharma and biopharma, drug discovery and development, target and lead identification and validation, G-protein coupled receptor biology, cell-based GPCR reporter assays
Gene Symbol CALCR2
Includes GeneBLAzer™ CALCR -CRE-bla FreeStyle™ 293F Cells (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: GeneBLAzer™ CALCR-CRE-bla Freestyle™ 293F cells, GeneBLAzer™ CALCR-CRE-bla Freestyle™ 293F Assay Protocol, Certificate of Analysis
Population Status Single Cell Clone Origin
Product Line GeneBLAzer
Quantity 2 x 106 Cells
Reporter Gene BLA (Beta-Lactamase)
Sample Type FreeStyle™ 293-F
Target Entry CALCR2
Type Cell Line
Cell Line FreeStyle 293-F Cells
System Type GeneBLAzer™
Druggable Target GPCR
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For Research Use Only. Not for use in diagnostic procedures.

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