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Thermo Scientific™ GeneBLAzer™ CALCRL:RAMP1-CRE-bla FreeStyle™ 293F Cells
Description
GeneBLAzer™ CALCRL:RAMP1-CRE-bla FreeStyle™ 293F cells contain the human Calcitonin Gene Related Peptide Receptor (CALCRL:RAMP1), (Accession No. NM_005795.3 NM_005855.2) stably integrated into the CellSensor™ CRE-bla Freestyle™ 293F cell line. CellSensor™ CRE-bla Freestyle™ 293F cells (Cat. No. K1130) contain a beta-lactamase (bla) reporter gene under control of the cAMP response element (CRE).
CALCRL:RAMP1-CRE-bla FreeStyle™ 293F cells are functionally validated for Z'-factor and EC50 concentrations of Alpha-CGRP. In addition, CALCRL:RAMP1-CRE-bla FreeStyle™ 293F cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section.
Specifications
Specifications
| Accession Number | NM_005855.2 |
| Assay Entry | Cell-based beta lactamase reporter gene |
| Cell State | Dividing Cells |
| Content And Storage | LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol. Includes: • GeneBLAzer™ CALCRL:RAMP1-CRE-bla Freestyle™ 293F cells • GeneBLAzer™ CALCRL:RAMP1-CRE-bla Freestyle™ 293F Assay Protocol • Certificate of Analysis The GeneBLAzer™ CALCRL:RAMP1-CRE-bla Freestyle™ 293F cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt. |
| Description | GeneBLAzer™ CALCRL:RAMP1-CRE-bla FreeStyle™ 293F Cells, 8 x 106 Cells, ∽8,000,000 cells/vial at 8,000,000 cells/mL Quantity of Cells, FreeStyle™ 293-F Cell Line, Fluorescent Detection Method, GPCRs Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage |
| Detection Method | Fluorescence |
| For Use With (Application) | Pharma and biopharma, drug discovery and development, target and lead identification and validation, G-protein coupled receptor biology, cell-based GPCR reporter assays |
| Gene Symbol | CALCRL:RAMP1 |
| Includes | GeneBLAzer™ CALCRL:RAMP1-CRE-bla FreeStyle™ 293F Cells (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: GeneBLAzer™ CALCRL:RAMP1-CRE-bla Freestyle™ 293F cells, GeneBLAzer™ CALCRL:RAMP1-CRE-bla Freestyle™ 293F Assay Protocol, Certificate of Analysis |
| Population Status | Single Cell Clone Origin |
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For Research Use Only. Not for use in diagnostic procedures.
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