missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Cell Analysis Products
  4. Cell Based Assays
  5. General Cell Based Assays
  6. 10267413

Thermo Scientific™ GeneBLAzer™ EDG7-NFAT-bla HEK 293T Cells

Product Code. 10267413
Click to view available options
Quantity:
2 x 106 Cells
Unit Size:
Each
This item is not returnable. View return policy

Product Code. 10267413

Brand: Thermo Scientific™ K1511

Please to purchase this item. Need a web account? Register with us today!

This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
 View alternative products

This item is not returnable. View return policy

Description

GeneBLAzer™ EDG7-NFAT-bla HEK293T cells contain the human Endothelial Differentiation Gene (EDG7) stably integrated into the CellSensor™ NFAT-bla HEK293T cell line.

GeneBLAzer™ EDG7-NFAT-bla HEK293T cells contain the human Endothelial Differentiation Gene (EDG7) stably integrated into the CellSensor™ NFAT-bla HEK293T cell line. CellSensor™ NFAT-bla HEK293T cells (Cat. No. K1538) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element.

The GeneBLAzer™ EDG7-NFAT-bla HEK293T cells are functionally validated for Z' and EC50 concentrations of 18:1 LPA. In addition, GeneBLAzer™ EDG7-NFAT-bla HEK293T cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

LPA receptors were originally classified in the Endothelial Differentiation Gene (EDG) family of receptors. The EDG family was later divided into two distinct groups of receptors based on their ligand specificity for either Lysophosphatidic acid (LPA) or Sphingosine-1-Phosphate (S1P). There are three high affinity LPA receptors LPA1, LPA2, and LPA3 (formally EDG2, EDG4, and EDG7), and five high affinity S1P receptors S1P1, S1P2, S1P3, S1P4 and S1P5 (formally EDG1, EDG5, EDG3, EDG6, and EDG8). LPA receptors are differentially linked via Gi and Gq to multiple effector systems and diverse biological functions. Recent studies revealed that targeted deletion of the EDG7/LPA3 receptor results in delayed implantation and altered embryo spacing, and significantly reduced litter size in mice. These findings indicate that EDG7 plays a crucial role in implantation and mammalian reproduction.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

TRUSTED_SUSTAINABILITY

Specifications

Accession Number NM_012152.1
Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.

Includes:
• GeneBLAzer™ EDG7-NFAT-bla HEK293T cells
• GeneBLAzer™ EDG7-NFAT-bla HEK293T Assay Protocol
• Certificate of Analysis

Storage:
The GeneBLAzer™ EDG7-NFAT-bla HEK293T cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method Fluorescence
For Use With (Application) FRET
Gene Symbol EDG7
Population Status Single Cell Clone Origin
Product Line GeneBLAzer
Quantity 2 x 106 Cells
Reporter Gene BLA (Beta-Lactamase)
Target Entry EDG7
Cell Line 293T (HEK)
System Type GeneBLAzer™
Druggable Target GPCR
Show More Show Less

For Research Use Only. Not for use in diagnostic procedures.

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.