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Thermo Scientific™ GeneBLAzer™ HCRTR1-NFAT-bla CHO-K1 Cells

Product Code. 10297463
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Product Code. 10297463 Supplier Thermo Scientific™ Supplier No. K1446

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Description

The GeneBLAzer™ HCRTR1-NFAT-bla CHO-K1 cells contain the human Hypocretin (Orexin) Receptor 1 (HCRTR1), stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line.

The GeneBLAzer™ HCRTR1-NFAT-bla CHO-K1 cells contain the human Hypocretin (Orexin) Receptor 1 (HCRTR1), (Accession No. NM_001525) stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line. CellSensor™ NFAT-bla CHO-K1 cells (Cat. No. K1078) contain a beta-lactamase (bla) reporter gene under control of the NFAT Response Element.

The HCRTR1-NFAT-bla CHO-K1 cells are functionally validated for Z'-factor and EC50 concentrations of Orexin A. In addition, HCRTR1-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section.

TRUSTED_SUSTAINABILITY

Specifications

Accession Number BC074796.2
Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.

Includes:• GeneBLAzer ™ HCRTR1 -NFAT-bla CHO-K1 cells
• GeneBLAzer ™ HCRTR1 -NFAT-bla CHO-K1 Assay Protocol
• Certificate of Analysis

The GeneBLAzer™ HCRTR1 -NFAT-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Description GeneBLAzer™ HCRTR1-NFAT-bla CHO-K1 Cells, 2 x 106 Cells, ∽2,000,000 cells/vial at 2,000,000 cells/mL Quantity of Cells, CHO-K1 Cell Line, Fluorescent Detection Method, GPCRs Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage
Detection Method Fluorescence
For Use With (Application) Pharma and biopharma, drug discovery and development, target and lead identification and validation, G-protein coupled receptor biology, cell-based GPCR reporter assays
Gene Symbol HCRTR1
Includes GeneBLAzer™ HCRTR1-NFAT-bla CHO-K1 Cells (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: GeneBLAzer™ HCRTR1 -NFAT-bla CHO-K1 cells, GeneBLAzer ™ HCRTR1
Population Status Pool of Stable Cells
Product Line GeneBLAzer
Quantity 2 x 106 Cells
Reporter Gene BLA (Beta-Lactamase)
Sample Type CHO-K1
Target Entry HCRTR1
Type CHO-K1 Cells
Cell Line CHO-K1
System Type GeneBLAzer™
Druggable Target GPCR
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For Research Use Only. Not for use in diagnostic procedures.

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