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Description
Gibco High-Intensity Perfusion (HIP) CHO Medium is an innovative, chemically defined, animal origin-free (AOF), cGMP medium. This easy-to-use perfusion medium helps enable high titers in numerous CHO cell lines from the beginning to the end of the process regardless of the type of perfusion platform, all while using less medium.
Features of HIP CHO Medium include:
- Tested with CHO-S, CHO-DG44, CHO-GS, and CHO-K1 cell lines
- Supports high cell density of over 100 million cells/mL at 1 vessel volume/day (VVD) continuous perfusion
- Demonstrated production of up to 1.7 g/L/day at 1 VVD continuous perfusion
Flexibility
HIP CHO Medium can be used at multiple concentrations to support seed train to production with one product. The flexible reconstitution of the medium allows dynamic tailoring of medium concentration to meet process and clone needs. Just as it can be used at multiple concentrations, HIP CHO Medium can be used in multiple perfusion processes: N-1 and seed train, intensified fed-batch, concentrated fed-batch, and continuous perfusion.In addition, HIP CHO Medium supports high cell densities at low medium exchange rates (as low as 1 VVD), which improves process efficiency.
HIP CHO Medium can help enable your move toward perfusion processes, which in turn can lead to a more compact and efficient workflow while improving productivity and quality.
Applications of HIP CHO Medium:
- Suspension CHO cells
- Selection system such as glutamine synthetase (GS) and dihydrofolate reductase (DHFR)
- Passaging, seed train, and protein production applications
- Optimized for use without feeds in perfusion application (other than glucose)
Specifications
Specifications
| Cell Line | CHO-S, CHO-GS, CHO-K1, CHO-DG44 |
| Concentration | 34.92 g/L |
| Form | Dry Powder |
| Product Type | Perfusion CHO Medium |
| Sterility | Non-sterile |
| With Additives | Glucose |
| Without Additives | No Glutamine |
| Manufacturing Quality | cGMP |
| Quantity | 15 L |
| Shipping Condition | Wet Ice |
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Frequently Asked Questions (FAQs)
In general, High-Intensity Perfusion (HIP) CHO Medium was not intended to be paired with concentrated feeds and as such may not benefit from them the way that other media intended for fed-batch application do.
Cells that are grown in or adapted to, and frozen in Gibco media such as CD CHO, CH OptiCHO, FortiCHO, Dynamis, and ExpiCHO seem to readily adapt to High-Intensity Perfusion (HIP) CHO Medium. However, adaptation should always be first demonstrated in flasks as suggested in the product manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0019412_High-IntensityPerfusionCHO_Medium_UG.pdf). Only when it is proven that a cell clone suffers no growth lag should it be thawed directly into HIP CHO Medium and/or switched into HIP CHO Medium mid-process workflow.
When using a CHO cell line with a selection system other than the glutamine synthetase (GS) system, we recommend adding 2-8 mM L-Glutamine or GlutaMAX Supplement to High-Intensity Perfusion (HIP) CHO Medium. Additionally, we recommend making sure that glucose levels do not fall below 2 g/L in culture by supplementing with glucose as needed. Also, if clumping becomes an issue, then anti-clumping agent (ACA) can be used.
Fed-batch oriented formulations typically perform best when paired with a feed. High-Intensity Perfusion (HIP) CHO Medium, by comparison, is formulated at a higher native concentration and assumes constant medium exchange without the addition of other feeds. Further, the osmolality of HIP CHO Medium at standard concentration is much higher than that of a typical fed-batch medium with the understanding that typical perfusion results in a lower osmolality. Lastly, HIP CHO Medium supports being reconstituted at multiple concentrations to better support the wide array of perfusion operating environments and seed train vs production needs.
There are many situations where you may want to consider using High-Intensity Perfusion (HIP) CHO Medium:
- Want to switch to perfusion with transfected CHO suspension culture
- Want a single platform with minimal complexity
- Desire high process flexibility
Additionally, HIP CHO medium allows sustained continuous production perfusion for long duration.
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