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Thermo Scientific™ SuperSignal™ West HisProbe Kit

Product Code. 10573364 Shop All Thermo Scientific Products
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685 mL
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1 set
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10573364 685 mL 1 set
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Product Code. 10573364 Supplier Thermo Scientific™ Supplier No. 15168

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Beschreibung

Directly detect histidine-tagged fusion proteins and other histidine-rich proteins with this nickel-activated horseradish peroxidase (HRP) derivative.

Thermo Scientific™ HisProbe-HRP is an nickel (Ni2+)-activated derivative of horseradish peroxidase (HRP). This product has been optimized for direct detection of recombinant histidine-tagged proteins and other histidine-rich proteins.

Unlike anti-6xHis antibodies, HisProbe-HRP can recognize polyhistidine tags independent of adjacent tags. The detection kit includes blocking buffers and chemiluminescent substrate for sensitive Western blot detection via the HisProbe-HRP Conjugate. The active ligand in HisProbe-HRP is a tridentate chelator that allows Ni2+ to be bound in active form for subsequent interaction and detection fo target molecules. The active chelator has binding capabilities similar to iminodiacetic acid (IDA) when used for immobilized metal affinity chromatography (IMAC).

Highlights:

  • Fast – His-tag detection is achieved with a one-step probe incubation that eliminates the need to run a lengthy two-step primary/secondary antibody reaction protocol

  • Economical – no need to purchase expensive primary/secondary antibodies; considerably less costly than anti-6xHis-tag antibodies

  • High specificity – HisProbe-HRP can recognize his-tags independent of adjacent tags

  • Less steric hindrance – HisProbe-HRP is smaller than antibodies and detects his tagged fusion proteins that are often undetectable when using some anti-his-tag antibodies

TRUSTED_SUSTAINABILITY

Spezifikation

Description Pierce SuperSignal West HisProbe Kit
Product Type HisProbe Detection Kit
Detection Method Chemiluminescence
For Use With (Application) Western Blot
Membrane Compatibility Nitrocellulose, PVDF
Product Line HisProbe, SuperSignal
Quantity 685 mL
Conjugate HRP
Substrate Horseradish Peroxidase
Reactivity All species
Sufficient For 62 mini blots or 5000 sq cm membrane
Content And Storage Sufficient For: 62 mini blots or 5000 cm2 membrane
• HisProbe-HRP Conjugate, 2 mg
• SuperSignal West Pico Luminol/Enhancer Solution, 250 mL
• SuperSignal West Pico Stable Peroxide Solution, 250 mL
• Blocker BSA in TBS (10X), 125 mL
• TBS Packs (each makes 500 mL), 10 packs
• Surfact-Amps 20 Detergent Solution, 6 x 10 mL

Store HisProbe-HRP, Blocker BSA in TBS and Surfact-Amps 20 at 4°C. Store remaining components at 4°C.
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For Research Use Only. Not for use in diagnostic procedures.

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