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Cytiva HiTrap™ Protein A HP Prepacked Columns

Product Code. 15157946 Shop All Cytiva Products
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Quantity:
1 x 1 mL
1 x 5 mL
2 x 1 mL
5 x 1 mL
5 x 5 mL
Flow Rate:
<20 mL/min.
<4 mL/min.
Unit Size:
1mL
Each
Pack of 2
Pack of 5
Product Code. Quantity Flow Rate unitSize
15157946 1 x 1 mL <4 mL/min. 1mL
11768438 5 x 5 mL <20 mL/min. Pack of 5
10676315 5 x 1 mL <4 mL/min. Pack of 5
10146184 2 x 1 mL <4 mL/min. Pack of 2
11758438 1 x 5 mL <20 mL/min. Each
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Product Code. 15157946

Brand: Cytiva 29048576

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Description

Use for purification and isolation of monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants. Cytiva Life Sciences™ HiTrap™ Protein A HP Prepacked Columns are specially designed, together with the medium to provide fast, simple and easy separations in a convenient format.

  • Prepacked with Protein A Sepharose High Performance media for purification and fractionation of IgG subclasses
  • For use with syringe, pump or chromatography system such as KTA design or FPLC System
  • Binds 20mg human IgG/mL medium
  • Stable over pH 3 to 9 for use and 2 to 10 for cleaning
  • Designed for use with a syringe, a peristaltic pump, or a chromatography system
  • Maximum pressure: 3 bar
  • Column material: polypropylene
  • 1 and 5mL columns

Media
  • Ligand: Native protein A
  • Matrix: 6% cross-linked agarose
  • Average particle size: 34μm
  • pH stability working range: 3 to 9

1mL columns

  • Bed dimensions: 7 x 25mm
  • Flow velocity: <4mL/min.

5mL columns
  • Bed dimensions: 16 x 25mm
  • Flow velocity: <20mL/min.

TRUSTED_SUSTAINABILITY

Specifications

Binding Capacity 20 mg/mL IgG Medium
Flow Rate <4 mL/min.
For Use With (Application) Chromatography System, Manual
For Use With (Equipment) For syringe, peristaltic pump or a chromatography system
Content And Storage 4°C to 8°C, 20% Ethanol
Quantity 1 x 1 mL

Safety and Handling

compliance-icons
Product Identifier
  • HiTrap Protein A HP 1 ml
 Signal Word
  • Warning
 Hazard Category
  • Flammable liquid Category 3
 Hazard Statement
  • H225-Highly flammable liquid and vapour.
 Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : ethanol
  • UNKNOWN ACUTE-16.5 per cent of the mixture consists of component(s) of unknown acute toxicity.
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