missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Chromatography Supplies
  4. Chromatography Media
  5. Cytiva HiTrap™ TALON™ Crude Prepacked Columns

Cytiva HiTrap™ TALON™ Crude Prepacked Columns

Product Code. 11618893 Shop All Cytiva Products
Click to view available options
Quantity:
1 x 1 mL
5 x 1 mL
5 x 5 mL
Unit Size:
1mL
Pack of 5
This item is not returnable. View return policy

Product Code. 11618893

Brand: Cytiva 28953766

Please to purchase this item. Need a web account? Register with us today!

Edge
This item is not returnable. View return policy

Description

For purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC). Cytiva Lifescience™ HiTrap™ TALON™ Crude Prepacked Columns offer a cobalt-based medium allowing protein purification under native or denaturing conditions.

Cobalt-based IMAC medium offering different selectivity compared to nickel-charged media. Allows protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.

  • Prepacked with cobalt-based chromatography media
  • Binding capacity approximately 7mg MBP
  • Maximum pressure: 3 bar
  • Column material: polypropylene
  • Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH
  • 1 and 5mL columns

Media
  • Ligand: TALON™ Superflow Cobalt-IMAC
  • Matrix: 6% cross-linked agarose
  • Average particle size: 60 to 160μm
  • pH stability working range: 3 to 12

1mL columns

  • Column I.D.: 7mm
  • Flow velocity: 1 to 4mL/min.

5mL columns
  • Column I.D.: 16
  • Flow velocity: 5 to 20mL/min.

TRUSTED_SUSTAINABILITY

Specifications

Quantity 5 x 1 mL
For Use With (Application) For PurifiCatIon of Histidine-Tagged Recombinant Proteins by Immobilized Metal Affinity Chromatography, Used Buffers and Easily Regenerated with 0.5 M NaOH
Storage Requirements 4°C to 8°C
Binding Capacity Up to 20 mg Histidine-tagged Protein1
Diameter (English) Inner 0.27 in.
Product Type HiTrap Talon crude prepacked Column
Diameter (Metric) Inner 0.7 cm
Flow Rate 1 to 4 mL/min
Particle Size 60 to 160 μm
Material Polypropylene
Max. Pressure 3 bar
Matrix 6% Cross-Linked Agarose
pH Range 3 to 12
Storage Buffer 20% Ethanol
Ligand Type Talon Superflow Cobalt-IMAC
Packaging Quantity 5 x 1 mL
Show More Show Less

Safety and Handling

compliance-icons
Signal Word
  • Warning
 Hazard Category
  • Flammable liquid Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • EUH208-Contains cobalt. May produce an allergic reaction.
 Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P235-Keep cool.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : Ethanol, Methanol, Cobalt
Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.