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Invitrogen™ Human ATP6V0A4 (aa 111-170) Control Fragment Recombinant Protein

Product Code. 30211417
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Quantity:
100 μL
Unit Size:
100µL
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Product Code. 30211417

Brand: Invitrogen™ RP103564

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This item is not returnable. View return policy

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (83%), Rat (83%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-84650 (PA5-84650. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', and d. This gene is one of four genes in man and mouse that encode different isoforms of the a subunit. Alternatively spliced transcript variants encoding the same protein have been described. Mutations in this gene are associated with renal tubular acidosis associated with preserved hearing. [provided by RefSeq, Jul 2008]
TRUSTED_SUSTAINABILITY

Specifications

Accession Number Q9HBG4
Concentration ≥5.0 mg/mL
For Use With (Application) Blocking Assay, Control
Formulation 1 M urea, PBS with no preservative; pH 7.4
Gene ID (Entrez) 50617
Name Human ATP6V0A4 (aa 111-170) Control Fragment
Quantity 100 μL
Regulatory Status RUO
Gene Alias a4; Atp6n1b; ATP6N2; Atp6v0a4; ATPase H+ transporting V0 subunit a4; ATPase, H+ transporting, lysosomal (vacuolar proton pump) noncatalytic accessory protein 1 B; ATPase, H+ transporting, lysosomal (vacuolar proton pump) non-catalytic accessory protein 1 B; ATPase, H+ transporting, lysosomal (vacuolar proton pump) non-catalytic accessory protein 2 (38 kD); ATPase, H+ transporting, lysosomal V0 subunit A4; H(+)-transporting two-sector ATPase, noncatalytic accessory protein 1 B; RDRTA2; RTA1C; RTADR; STV1; tcag7.346; vacuol; vacuolar proton pump 116 kDa accessory subunit; vacuolar proton pump, subunit 2; vacuolar proton translocating ATPase 100 kDa a4 subunit; Vacuolar proton translocating ATPase 116 kDa subunit a isoform 4; Vacuolar proton translocating ATPase 116 kDa subunit a kidney isoform; V-ATPase 116 kDa; V-ATPase 116 kDa isoform a4; V-ATPase alpha 4; VPH1; VPP2; V-type proton ATPase 116 kDa subunit a; V-type proton ATPase 116 kDa subunit a isoform 4
Common Name ATP6V0A4
Gene Symbol ATP6V0A4
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Protein Tag His-ABP-tag
Sequence NQNQQALKQSFLELTELKYLLKKTQDFFETETNLADDFFTEDTSGLLELKAVPAYMTGKL
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Expression System E. coli
Form Liquid
Purity or Quality Grade >80% by SDS-PAGE and Coomassie blue staining
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