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Proteintech™ Human Granzyme B ELISA Kit

Product Code. 16891526 Shop All Proteintech Products
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Target Species:
Human
Unit Size:
96 tests
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Product Code. Target Species unitSize
16891526 Human 96 tests
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Artikelnummer. 16891526 missing translation for 'mfr' Proteintech™ missing translation for 'supplierNo' KE0012196T

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Beschreibung

Solid phase sandwich ELISA used to detect and quantify protein levels of endogenous Granzyme B

  • Recognizes human granzyme B
  • Granzyme B (GZMB, CG1, CSPB, CTLA1, GRB) belongs to granzyme subfamily
    • Enzyme necessary for target cell lysis in cell-mediated immune responses
    • Cytotoxic lymphocyte protease GZMB can promote apoptosis through direct processing and activation of members of caspase family
    • Functions as processing protease of cytokines (interleukin, IL-18, IL-1a) and extracellular matrix proteins (fibronectin) and proteoglycans
    • Plasma granzyme B concentrates significantly higher in patients with AD and psoriasis
      • Granzyme B may play role in induction of intractable itch and dermatitis in AD

Process:

  • Anitbody specific for granzyme B pre-coated onto microwells
  • Granzyme B protein in samples captured by the coated antibody after incubation
  • After extensive washing, another antibody of biotinylated specific for granzyme B is added to detect captured granzyme B protein
  • For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent
  • Solution containing sulfuric acid used to stop color development and color intensity
    • Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm

Spezifikation

Accession Number BC030195
Assay Range 15.6 to 1000 pg/mL
Assay Sensitivity 2.6 pg/mL
Certifications/Compliance ISO 13485
Detection Method Sandwich ELISA, Double Antibody
Immunoassay Kit Format Coated ELISA Kit
Sample Type Plasma, Serum
Substrate DAB
Target Species Human
Gene ID (Entrez) 3002
Host Species Human
Immunoassay Kit Grade Solid
Interassay CV <10%
Intraassay CV <10%
Shipping Condition Blue ice
Storage Requirements -20°C to +4°C
Total Assay Time 4.5 hr.
UniProt ID P10144
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Sicherheit und Handhabung

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Produktidentifikator
  • 96TEST Human Granzyme B ELISA Kit
 Signalwort
  • Warning
 Gefahrenkategorie
  • Serious eye damage/eye irritation Category 1
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Gefahrenhinweis
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H318-Causes serious eye damage.
  • H319-Causes serious eye irritation.
  • H332-Harmful if inhaled.
  • H335-May cause respiratory irritation.
 Sicherheitshinweis
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P234-Keep only in original container.
  • P264-Wash skin thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P330-Rinse mouth.
  • P362-Take off contaminated clothing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Ergänzende Informationen
  • MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine
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