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  5. Invitrogen™ Human MMP-12 ELISA Kit

Invitrogen™ Human MMP-12 ELISA Kit

Product Code. 17164623
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Quantity:
10 x 96 Tests
5 x 96 Tests
96 Tests
Unit Size:
Each
Pack of 10
Pack of 96
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Product Code. 17164623

Brand: Invitrogen™ EH327RB

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Description

ELISA

Human MMP-12 quantitates human MMP-12 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human MMP-12.

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. The gene is part of a cluster of MMP genes which localize to chromosome 11q22. 3.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number 3.4.24.65; AV378681; HME; Macrophage elastase; Macrophage metalloelastase; macrophage-metalloelastase; matrix metallo protease; matrix metallopeptidase 12; matrix metallopeptidase 12 (macrophage elastase); matrix metalloproteinase 12; matrix metalloproteinase 12 (macrophage elastase); matrix metalloproteinase-12; ME; MGC138506; Mme; Mmel; MMP; MMP12; MMP-12; MMPs
Assay Range 0.04 to 10 ng/mL
Assay Sensitivity 0.04 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 4321
Gene Symbol Mmp12
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target MMP12
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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Safety and Handling

compliance-icons
Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
 Hazard Statement
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
 Precautionary Statement
  • P264-Wash thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P302+P352-IF ON SKIN: Wash with plenty of water/.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P332+P313-If skin irritation occurs: Get medical advice/attention.
  • P337+P313-If eye irritation persists: Get medical advice/attention.
  • P362+P364-Take off contaminated clothing and wash it before reuse.
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