missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Recombinant Proteins
  4. Invitrogen™ Human TNF-alpha Recombinant Protein

Invitrogen™ Human TNF-alpha Recombinant Protein

Product Code. 16627273
Click to view available options
Quantity:
5 μg
5 x 5 μg
50 μg
Unit Size:
50µg
5µg
Pack of 5
Este artículo no se puede devolver. Vea la política de devoluciones

Código de producto. 16627273

Marca: Invitrogen™ A42551

Please to purchase this item. Need a web account? Register with us today!

Este artículo no se puede devolver. Vea la política de devoluciones

Descripción

Recombinant Protein

Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
TRUSTED_SUSTAINABILITY

Especificaciones

Accession Number P01375
For Use With (Application) Control, Bioactivity, Immunohistochemistry, Surface Plasmon Resonance, Western Blot
Formulation PBS with 5% mannitol, 5% trehalose, 0.01% Tween 80 and no preservative; pH 7.4
Gene ID (Entrez) 7124
Molecular Weight (g/mol) 17.4 kDa
Name Human TNF-alpha
pH Range 7.4
Purification Method Purified
Quantity 5 μg
Source E. coli
Storage Requirements -20°C or -80°C if preferred
Regulatory Status RUO
Gene Alias APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF α; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFα; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor
Common Name TNF alpha
Gene Symbol Tnf
Biological Activity 1.Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL 2.Captured Adalimumab on Anti-human IgG Fc via CM5 Chip can Human TNF-alpha Recombinant Protein with an affinity constant of 0.2843 nM as determined in a SPR assay (Biacore T200)(Routinely tested).
Product Type Protein
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Protein Tag Untagged
Sequence Human TNF-alpha, amino acids Val77-Leu233 (Accession # NP_000585.2) with an N-terminal Met
Content And Storage Includes 1 vial of lyophilized protein. Store at -20 °C.
Activity 1.Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL.<br><br>2.Captured Adalimumab on Anti-human IgG Fc via CM5 Chip can Human TNF-alpha Recombinant Protein with an affinity constant of 0.2843 nM as determined in a SPR assay (Biacore T200)(Routinely tested).
Expression System E. coli
Protein Family Cytokines & Receptors
Protein Form Full Length, Recombinant
Form Lyophilized
Protein Subtype TNF (Tumor Necrosis Factor)
Research Category Immunology, Inflammation, Oncology, Signal Transduction
Purity or Quality Grade ≥95 % as determined by SDS-PAGE. ≥95 % as determined by SEC-HPLC.
Protein tumor necrosis factor
Mostrar más Mostrar menos
Corrección del contenido de un producto

Proporcione sus comentarios sobre el contenido del producto rellenando el siguiente formulario.

Título del producto

Al hacer clic en Enviar, acepta que Fisher Scientific se ponga en contacto con usted en relación con los comentarios que ha proporcionado en este formulario. No compartiremos su información para ningún otro fin. Toda la información de contacto proporcionada se mantendrá de acuerdo con nuestra Política de Privacidad. Política de privacidad.

Gracias por ayudarnos a mejorar nuestra web. Su comentario ha sido enviado