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Proteintech™ Human VEGFR-1 ELISA Kit

Product Code. 16811526 Shop All Proteintech Products
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Target Species:
Human
Unit Size:
96 tests
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Product Code. 16811526

Brand: Proteintech™ KE0011396T

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This item is not returnable. View return policy

Description

KE00113 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The VEGFR-1 ELISA kit is to be used to detect and quantify protein levels of endogenous VEGFR-1.

  • The assay recognizes human VEGFR-1. An antibody specific for VEGFR-1 has been pre-coated onto the microwells. The VEGFR-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for VEGFR-1 is added to detect the captured VEGFR-1 protein.
  • For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
  • Vascular endothelial growth factor receptor-1 (VEGFR-1, FLT-1) is a receptor tyrosine kinase belonging to the VEGFR family. VEGF is a key regulator of physiological angiogenesis and has also been implicated in pathological angiogenesis associated with tumors, intraocular neovascular disorders and other conditions.
  • The biological effects of VEGF are mediated by VEGFR-1 and VEGFR-2. Both the two receptors have seven immunoglobulin-like repeats in the extracellular domain, a single transmembrane region and a tyrosine kinase domain.
  • VEGFR-1 binds VEGFA, PIGF and VEGFB, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. Soluble VEGF receptor-1 (sVEGFR-1, sFlt-1), a truncated version of the cell membrane-spanning VEGFR-1, has the potential to act as a decoy receptor for VEGFA.
  • Elevated serum sVEGFR-1 have been found in women with preeclampsia. Excess placental sVEGFR-1 may contribute to endothelial dysfunction, hypertension, and proteinuria in preeclampsia.

Specifications

Accession Number BC039007
Assay Range 62.5 to 4000 pg/mL
Assay Sensitivity 7.8 pg/mL
Certifications/Compliance ISO 13485
Detection Method Sandwich ELISA, Double Antibody
Immunoassay Kit Format Coated ELISA Kit
Sample Type Plasma, Serum
Substrate DAB
Target Species Human
Gene ID (Entrez) 2321
Host Species Human
Immunoassay Kit Grade Solid
Interassay CV <10%
Intraassay CV <10%
Shipping Condition Blue ice
Storage Requirements -20°C to +4°C
Total Assay Time 4.5 hr.
UniProt ID P17948
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Safety and Handling

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Product Identifier
  • 96TEST Rat TGF-beta1 ELISA Kit
 Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 1
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H318-Causes serious eye damage.
  • H319-Causes serious eye irritation.
  • H332-Harmful if inhaled.
  • H335-May cause respiratory irritation.
 Precautionary Statement
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P234-Keep only in original container.
  • P264-Wash skin thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P330-Rinse mouth.
  • P362-Take off contaminated clothing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine
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