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  5. Invitrogen™ Rat ICAM-1 ELISA Kit

Invitrogen™ Rat ICAM-1 ELISA Kit

Product Code. 15415383
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5 x 96 Tests
96 Tests
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1 set
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15415383 96 Tests 1 set
17175919 5 x 96 Tests Each
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Product Code. 15415383 Supplier Invitrogen™ Supplier No. ERICAM1

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Description

ELISA

The Rat InterCellular Adhesion Molecule 1 (Rt ICAM-1) ELISA quantitates Rt ICAM-1 in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt ICAM-1. Principle of the method The Rat ICAM-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number Q00238
Assay Range 28.81 to 7000 pg/mL
Assay Sensitivity 20 pg/ml
Calibration Range 20 pg/mL to 7000 pg/mL
Assay Solid-phase sandwich ELISA
Conjugate HRP
Product Type ELISA
Protein Family Chemokine & Receptors
Sample Type Plasma, Serum, Supernatant
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta- NGF, TIMP-1, TNF-alpha.
Sufficient For 96 assays
Target Species Rat
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 25464
Gene Symbol CD54,ICAM
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Inflammation
Sample Volume Plasma 5 μL, Serum 5 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Synonym CD54, ICAM
Target ICAM-1 (CD54)
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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