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  5. 11534955

Cytiva IMAC Sepharose™ High Performance Media

Product Code. 11534955 Shop All Cytiva Products
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Quantity:
100 mL
Unit Size:
100mL
Product Code. Quantity unitSize
11534955 100 mL 100mL
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Regulatory: This product contains a substance listed in Annex XVII to REACH Regulation (EC) No 1907/2006, all customers must complete a declaration before delivery.

Product Code. 11534955

Brand: Cytiva 17092007

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This item is not returnable. View return policy
Regulatory: This product contains a substance listed in Annex XVII to REACH Regulation (EC) No 1907/2006, all customers must complete a declaration before delivery.

Description

For optimizing purification of histidine-tagged proteins using Immobilized Metal Affinity Chromatography (IMAC) when Ni2+ is not the best choice of metal ion

  • 34μm beads of highly cross-linked 6% agarose, to which a chelating group has been covalently coupled
  • Charge with transition metal of choice for optimized selectivity
  • Small bead size for high performance purification
  • High binding capacity: ≥40mg histidine-tagged protein/mL medium when charged with Ni2+
  • Allows more sample to be applied; a more concentrated target protein is obtained more at a lower cost/mg and in less time
  • Stable in pH range 3-12 (long term), and in all commonly used buffers

Avoid use with chelating agents, e.g., EDTA, EGTA, or citrate.

TRUSTED_SUSTAINABILITY

Specifications

Flow Rate <300 cm/hr.
pH Range 3 to 12
Particle Size 34 μm
Product Type IMAC Sepharose HP
Content And Storage 4°C to 30°C
Quantity 100 mL
For Use With (Application) Purification of Polyhistidine tagged Proteins

Safety and Handling

compliance-icons
Product Identifier
  • IMAC sepharose high perfomance
 Signal Word
  • Warning
 Hazard Category
  • Flammable liquid Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
 Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : ethanol
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