missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Antibody Production and Purification
  5. Protein Immunogens
  6. 11894035

Thermo Scientific™ Imject™ Maleimide-Activated mcKLH Spin Kit

Product Code. 11894035 Shop All Thermo Scientific Products
Change view
Click to view available options
Quantity:
5 x 2 mg
Unit Size:
1 set
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity unitSize
11894035 5 x 2 mg 1 set
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
This item is not returnable. View return policy
Regulatorischer Hinweis: Dieses Produkt enthält Substanzen aus dem Anhang XVII der Verordnung REACH (EG) Nr. 1907/2006. Alle Kunden müssen vor der Lieferung eine Erklärung ausfüllen.
Artikelnummer. 11894035 Lieferant Thermo Scientific™ Lieferanten-Nr. 77666

Please to purchase this item. Need a web account? Register with us today!

Dieser Artikel kann nicht zurückgegeben werden. Rückgaberichtlinie anzeigen
Regulatorischer Hinweis: Dieses Produkt enthält Substanzen aus dem Anhang XVII der Verordnung REACH (EG) Nr. 1907/2006. Alle Kunden müssen vor der Lieferung eine Erklärung ausfüllen.

Beschreibung

Perform hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens with this sulfhydryl-reactive mariculture KLH.

Thermo Scientific™ Imject Maleimide-Activated mcKLH is sulfhydryl-reactive immunogenic mariculture keyhole limpet hemocyanin (KLH) for hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens.

The high-quality mariculture KLH is rendered sulfhydryl-reactive by chemical modification with Sulfo-SMCC crosslinker, and the resulting maleimide-activated protein is then stabilized and lyophilized in phosphate buffer. Maleimide groups form covalent crosslinks with sulfhydryl (-SH) moieties on cysteine residues of peptides and other thiol-containing haptens. Numerous antigen molecules can be conjugated to each KLH molecule to make them highly immunogenic and increase the likelihood of eliciting immunized animals to produce high-titer antisera to the epitopes of interest. Select from among several package sizes of activated KLH protein, or choose the complete conjugation kit that includes optimized buffers and trouble-free spin desalting columns.

Highlights:

  • Convenient packages – choose stabilized, lyophilized Maleimide-Activated mcKLH or a complete immunogen conjugation kit

  • Optimized activation – specifications require that each lot is activated with more than 400 moles of sulhydryl-reactive maleimide per mole of KLH, ensuring consistently high levels of conjugation

  • Sulfhydryl-reactive – engineered for conjugating cysteine-containing peptide antigens to make effective immunogens for high-titer antibody production

  • Simple, rapid procedure – complete the hapten-carrier conjugation reaction in two hours to yield sufficient immunogen for several injections and booster immunizations of host animals

  • High-quality carrier protein – KLH is isolated from the mollusk Megathura crenulata (keyhole limpet); mcKLH is a high-quality form of KLH that is harvested from limpets grown in mariculture

  • Highly immunogenic – KLH has a high molecular mass (0.5 to 13 million daltons; aggregates of 350 and 390kDa subunits) and elicits a stronger immune response than BSA or ovalbumin

  • Excellent solubility – our preparations of mariculture KLH are more soluble than typical forms and we also offer a PEGylated form for even greater hydrophilicity and solubility

Includes:

Kit contains stabilized, lyophilized Maleimide-Activated mcKLH (in PBS with stabilizer), buffers and desalting columns to easily prepare ready-to-use immunogens for immunization

TRUSTED_SUSTAINABILITY

Spezifikation

Content And Storage Sufficient For: 5 conjugation reactions and complete immunogen preparations, each with up to 2 mg sulfhydryl-containing peptide
• Imject Maleimide-Activated mcKLH, 5 x 2 mg
• Imject Maleimide Conjugation Buffer, 30 mL
• Imject Purification Buffer Salts, 5 x 0.84 g
• Zeba Spin Desalting Columns, 7K MWCO, 2 mL, 5 columns

Store the Maleimide-Activated blue Carrier Protein at -20°C. Store remaining components at 4°C.
Description Imject Maleimide Activated mcKLH Spin Kit
Format Kit
Quantity 5 x 2 mg
Product Line Imject

For Research Use Only. Not for use in diagnostic procedures.

Name des Produkts
Wählen Sie ein Thema

Indem Sie auf Absenden klicken, erklären Sie sich damit einverstanden, dass Fisher Scientific sich mit Ihnen in Verbindung setzen kann, um Ihr Feedback in diesem Formular zu bearbeiten. Wir werden Ihre Informationen nicht für andere Zwecke weitergeben. Alle bereitgestellten Kontaktinformationen werden in Übereinstimmung mit unserer Datenschutzrichtlinie aufbewahrt. Datenschutzrichtlinie.