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Invitrogen™ Bluo-Gal
Beschreibung
Bluo-Gal (halogenated indolyl-β-galactoside) is an alternative histochemical substrate for β-galactosidase that produces a darker blue color than X-gal, the more frequently used substrate. As with X-gal, Bluo-Gal produces blue (lac+) bacterial colonies and blue (lac+) viral plaques. Bacteria containing active β-galactosidase produce blue colonies when grown on plates containing Bluo-Gal.
Bluo-Gal can also be used as a substrate with the streptavidin-β-galactosidase conjugate in both immunoblot and immunocytochemistry assays. In the presence of β-galactosidase, Bluo-Gal yields a blue precipitate, which is insoluble in alcohols and xylenes. Bluo-Gal is soluble in dimethylformamide.
Primary applications
• Blue-white screening (deeper blue as compared to X-gal)—detect functional β-galactosidase (reporter gene expression)
• Histochemical detection of β-galactosidase activity in fixed tissues
• Bluo-Gal overlay assay
Find the media format that fits your needs
• Ready-to-use media formulations are available, such as LB Broth (Cat. No. 10855001), Terrific Broth (Cat. No. A1374301), or S.O.C recovery medium (Cat. No. 15544034).
• IPTG (Cat. No.15529019) is available for blue-white screening.
Bestellinformation
Shipping Condition: Room Temperature
Spezifikation
Spezifikation
| Content And Storage | • Bluo-Gal, substrate for β-galactosidase (1 g) Store at –5°C to –30°C. |
| Description | Bluo-Gal, Cell-Permeant |
| Detection Method | Colorimetric |
| Format | Bottle |
| Test Type | Blue/White Colony Screening, Immunoblotting, Immunocytochemical Assays |
| Material | Halogenated Indolyl-β-galactoside Substrate |
| Quantity | 1 g |
| Shipping Condition | Approved for shipment at Room Temperature or on Wet Ice |
For Research Use Only. Not for use in diagnostic procedures.
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