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Invitrogen™ ChargeSwitch™ Plasmid ER Mini Kit

Product Code. 10653923
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Product Code. 10653923 Supplier Invitrogen™ Supplier No. CS10100

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Description

Includes

  • ChargeSwitch Magnetic Beads (25 mg/mL) in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20
  • RNase A (5 mg/mL in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
  • Resuspension Buffer (R4; 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
  • ChargeSwitch Lysis Buffer (L9)
  • ChargeSwitch Precipitation Buffer (N5)
  • ChargeSwitch Wash Buffer (W11)
  • ChargeSwitch Wash Buffer (W12)
  • ChargeSwitch Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
  • Endotoxin Reduction Reagent (D1; ETRR)

Requires

Magnetic rack

ChargeSwitch™ Plasmid ER Mini Kit uses magnetic bead-based technology to allow for rapid and efficient purification of plasmid DNA.

ChargeSwitch™ Plasmid ER Mini Kit uses magnetic bead-based technology to allow for rapid and efficient purification of plasmid DNA from 1-5 mL of fresh, overnight bacterial cultures without the use of chaotropic salts, organic solvents, or ethanol, to yield up to 20 μg of transfection-grade plasmid DNA in a total time of about 10 minutes after sample preparation.

  • Optimized for higher-purity DNA extraction
  • Improved performance with accompanying magnetic rack
  • Cleaner purified plasmid for optimal downstream performance
  • High quality, high yield plasmid DNA
  • Compatible with MagnaRack™ magnetic rack
  • Reliable performance in variety of downstream applications

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage

All components of the ChargeSwitch™ Plasmid ER (Endotoxin Reduced) Mini Kit are shipped at room temperature. Upon receipt, store components as follows: Store Precipitation Buffer (N5) at 4°C. Mix RNase A in Resuspension Buffer (R4) and store RNase A in Resuspension Buffer (R4) at 4°C. Store the remaining components at room temperature. All components are guaranteed stable for 6 months when stored properly.

Components:
• ChargeSwitch™ Magnetic Beads (25 mg/mL) in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20
• RNase A (5 mg/mL in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
• Resuspension Buffer (R4; 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
• ChargeSwitch™ Lysis Buffer (L9)
• ChargeSwitch™ Precipitation Buffer (N5)
• ChargeSwitch™ Wash Buffer (W11)
• ChargeSwitch™ Wash Buffer (W12)
• ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
• Endotoxin Reduction Reagent (D1; ETRR)
Format Kit
Isolation Technology ChargeSwitch Chemistry
Scale Mini
Sample Type Bacterial Culture
Final Product Type Plasmid DNA
For Use With (Application) Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
High-throughput Compatibility Not High-throughput Compatible (Manual)
Prep Scale < 100 μg (Small-Scale) Plasmid DNA
Product Line ChargeSwitch
Product Type Plasmid ER Mini Kit
Quantity 50 Preps
Shipping Condition Room Temperature
Target Plasmid DNA
Test Time 10 min.
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For Research Use Only. Not for use in diagnostic procedures.

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