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Invitrogen™ CIAP (Calf Intestinal Alkaline Phosphatase), 1 U/μL

Product Code. 10593263
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1000 U
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Product Code. 10593263 Supplier Invitrogen™ Supplier No. 18009027

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112.01 EUR valid until 2026-03-20
BEST PRICE on Promo! Use promo code "27968" to get your promotional price.
This item is not returnable. View return policy
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27968” to get your promotional price

Description

Phosphomonoesterase that removes 3' and 5' phosphates from DNA and RNA

  • Concentration: 20 units/μL
  • Source: Purified from calf intestinal mucosa
  • Performance and Quality Testing: Endodeoxyribonuclease, 3' exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency measured in a transformation assay
  • Unit Definition: One unit hydrolyzes 1μmol of 4-nitrophenyl phosphate in 1 min. at 37°C
  • Unit Reaction Conditions: 1M diethanolamine buffer, 10mM 4-nitrophenyl phosphate, 0.25mM MgCl2 (pH 9.8) in 900μL for 10 min. at 37°C

Dephosphorylation of 5'-phosphorylated termini of vector DNA to prevent self-ligation; Dephosphorylation of 5' termini of nucleic acids prior to forward reaction with kinase; Cloning; Restriction Enzyme Cloning

TRUSTED_SUSTAINABILITY

Specifications

Concentration 1 U/μL
Content And Storage Calf Intestinal Alkaline Phosphatase (1 unit/μL) is supplied with a vial of 10X dephosphorylation buffer [500 mM Tris-HCl (pH 8.5), 1 mM EDTA], vial of dilution buffer [50% glycerol, 25 mM Tris-HCl (pH 7.6), 1 mM MgCl2 , and 0.1 mM ZnCl2 ]. Store Calf Intestinal Alkaline Phosphatase at -20°C.
Shipping Condition Approved for shipment on Wet or Dry Ice
Enzyme CIP
Compatible Buffer Diethanolamine Buffer
Quantity 1000 U
Product Type Alkaline Phosphatase

For Research Use Only. Not for use in diagnostic procedures.

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