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Invitrogen™ CELLection™ Epithelial Enrich Dynabeads™

Product Code. 10291743
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Quantity:
5 mL
Unit Size:
Each
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Product Code. 10291743

Brand: Invitrogen™ 16203

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This item is not returnable. View return policy

Includes

5mL CELLection; Epithelial Enrich Dynabeads coated with a monoclonal antibody towards the human Epithelial Cell Adhesion Molecule (EpCam). 2 vials Releasing Buffer Component 1 (DNase I) and 2mL Relasing Buffer Component 2 are also supplied. Up to 2x109 cells can be processed with this kit.

Use this kit to obtain up to 5-log enrichment of human epithelial tumor cells, directly from whole blood, bone marrow or PBMC.

Use this kit to obtain up to 5-log enrichment of human epithelial tumor cells, directly from whole blood, bone marrow or PBMC. Tumor cell clumps can be enriched from bone marrow. Enriched cells are bead-free, viable and suitable for immunocytochemical staining or any other downstream application.

High enrichment of viable human tumor cells
• Isolate directly from whole blood, bone marrow or PBMC
• Viable cells for any cellular applications

Methodology: Add CELLection™ Dynabeads™ to the sample. The beads will bind the tumor cells after a short incubation. The bead-bound tumor cells are quickly separated on a magnet and subsequently released from the beads with the supplied Release Buffer. (CELLection™ Dynabeads™ contains a DNA linker that is cleaved by the DNase I in the Release Buffer.)

Note: If you want to deplete or use the isolated tumor cells for molecular applications, please use the equivalent product Dynabeads™ Epithelial Enrich (Cat. No. 161-02). This product contains the same antibody, but with no release of the cells from the beads after positive isolation.

Starting Samples: Whole blood, bone marrow, buffy coat, PBMC.

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Cell Type Tumor Cells, Epithelial Cells
Content And Storage Each Kit Contains:
• 5 mL CELLection™ Epithelial Enrich Dynabeads™ coated with a monoclonal antibody towards the human Epithelial Cell Adhesion Molecule (EpCam)
• 2 vials Releasing Buffer Component 1 (DNase I)
• 2 mL Releasing Buffer Component 2

Up to 2 x 109 cells can be processed with this kit. Store components at 2°C to 8°C.
Product Type Epithelial Enrichment Kit
Isolation Technology Positive Isolation
No. of Cells Processes ∼4 x 109 cells total
Output Viability >95%
Product Line CELLection, DYNAL, Dynabeads
Purity or Quality Grade Research Grade
Quantity 5 mL
Reactivity Human
Sample Type PBMC, Blood
Shipping Condition Room Temperature
Starting Material Cell No. 2 x 107 PBMCs per isolation
Target Species Human
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For Research Use Only. Not for use in diagnostic procedures.

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