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Invitrogen™ Dynabeads™ M-450 Tosylactivated

Product Code. 10662814
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10662814 5 mL Each
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To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27970” to get your promotional price
Product Code. 10662814 Supplier Invitrogen™ Supplier No. 14013
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This item is not returnable. View return policy
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27970” to get your promotional price

Includes

5mL beads at a concentration of 4 x 108 beads/mL in distilled water

Dynabeads™ M-450 Tosylactivated are 4.5 μm superparamagnetic beads containing surface tosyl groups.

Dynabeads™ M-450 Tosylactivated are 4.5 μm superparamagnetic beads containing surface tosyl groups. The beads covalently bind primary amino and sulfhydryl groups in antibodies to position them in the optimal orientation for cell-surface protein binding. Low background and covalent binding of the antibodies make these beads an excellent choice for cell separation applications when a downstream release from the beads is not required.

• Quickly deplete cells or positively isolate target cells for downstream molecular analysis
• Excellent for cell isolation procedures using your own unique antibody
• Several antibodies can be coupled on the same beads for non-cell-based applications, such as cell activation

Coupling procedure
Covalent coupling is performed overnight by incubating the antibody of choice with the Dynabeads™ M-450 Tosylactivated beads. Optimal coupling occurs at high pH (8.5–9.5) and at 37°C. For pH-labile antibodies, coupling can be performed in an alternative buffer at pH 7.4.

Not recommended for immunoprecipitation (IP)
These 4.5 μm Tosylactivated beads are not recommended for IP as they have a lower surface capacity per unit mass when compared to 1 μm and 2.8 μm Tosylactivated and Epoxy Dynabeads™.

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Cell Type All cells from all species
Content And Storage Contains: 5 mL beads at a concentration of 4 x 108 beads/mL in distilled water.
Storage: 2°C to 8°C.
Product Type Tosylactivated Bead
Isolation Technology Depletion, positive isolation for molecular applications
No. of Cells Processes ∼2 x 109 cells total
Output Viability >95%
Product Line DYNAL, Dynabeads
Purity or Quality Grade Research Grade
Quantity 5 mL
Reactivity All species
Sample Type PBMC, Tissue Digests, Blood
Shipping Condition Room Temperature
Starting Material Cell No. 1 x 107 cells per isolation
Target Species All species
Diameter (Metric) 4.5 μm
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For Research Use Only. Not for use in diagnostic procedures.

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