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Invitrogen™ Flp-In™ Complete System

Product Code. 10729364
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Quantity:
1 kit
Unit Size:
Each
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Product Code. 10729364

Brand: Invitrogen™ K601001

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Description

Includes

The Flp-In Complete System includes the Core Kit (Cat. No. K601002), 1g Zeocin antibiotic, and 1g hygromycin B. All vectors are supplied supercoiled and lyophilized.

Allows integration and expression of gene of interest in mammalian cells at specific genomic location

  • Introduce of Flp Recombination Target (FRT) site into genome of mammalian cell line of choice
  • Expression vector containing gene of interest is then integrated into genome via Flp recombinase-mediated DNA recombination at FRT site
  • Major components include Flp-In. target site vector, pFRT/lacZeo, for generation of host cell line containing integrated FRT site
  • Expression plasmid containing a FRT site linked to hygromycin resistance gene for Flp recombinase-mediated integration and selection of stable cell line expressing gene of interest under control of human cytomegalovirus (CMV) immediate-early enhancer/promoter
  • Flp recombinase expression plasmid, pOG44, for expression of Flp recombinase under control of human CMV promoter
  • Control expression plasmid containing chloramphenicol acetyl transferase (CAT) gene, which when cotransfected with pOG44 into the Flp-In. host cell line, expresses CAT

Bioproduction, Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Stable Cell Line Development, Targeted Integration

TRUSTED_SUSTAINABILITY

Specifications

Constitutive or Inducible System Constitutive
Delivery Type Transfection
Promoter CMV
Product Type Flp-In Complete System
Selection Agent (Eukaryotic) Zeocin™, Hygromycin
Content And Storage The Flp-In™ Complete System includes the Core Kit (K601002), 1 g Zeocin™ antibiotic, and 1 g hygromycin B. All vectors are supplied supercoiled and lyophilized. Store hygromycin at +4°C. Store all other components at -20°C. Zeocin™ antibiotic should be protected from exposure to light. All reagents are guaranteed stable for 6 months when properly stored.
Protein Tag Untagged
Cloning Method Restriction Enzyme/MCS
Quantity 1 kit
Vector pFRT
Product Line Flp-In, pcDNA
For Use With (Application) Stable Cell Line Development, Targeted Integration
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For Research Use Only. Not for use in diagnostic procedures.

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