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Invitrogen™ Flp-In™ Core System

Product Code. 10398502
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Product Code. 10398502 Supplier Invitrogen™ Supplier No. K601002

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Description

Includes

The Flp-In Core Kit includes 20μg each of pFRT/lacZeo, pOG44, pcDNA5/FRT, and pcDNA5/FRT/CAT (expression control), and 2μg each of forward and reverse sequencing primers.

Contains necessary vectors that allow integration and expression of gene of interest in mammalian cells at specific genomic location

  • For introduction of Flp Recombination Target (FRT) site into genome of mammalian cell line of choice
  • Expression vector containing gene of interest is then integrated into genome via Flp recombinase-mediated DNA recombination at FRT site

Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Targeted Integration

TRUSTED_SUSTAINABILITY

Specifications

Constitutive or Inducible System Constitutive
Delivery Type Transfection
Promoter CMV
Product Type Flp-In Core System
Selection Agent (Eukaryotic) Zeocin™
Content And Storage The Flp-In™ Core Kit includes 20 μg each of pFRT⁄lacZeo, pOG44, pcDNA™5⁄FRT, and pcDNA™5⁄FRT⁄CAT (expression control), and 2 μg each of forward and reverse sequencing primers. Store at -20C.
Protein Tag Untagged
Cloning Method Restriction Enzyme/MCS
Quantity 1 kit
Vector pFRT
Product Line Flp-In, pcDNA
For Use With (Application) Targeted Integration
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For Research Use Only. Not for use in diagnostic procedures.

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