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Invitrogen™ pAd/CMV/V5-DEST™ Gateway™ Vector Kit

Product Code. 11540836
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Quantity:
6 μg
Unit Size:
6µg
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11540836 6 μg 6µg
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Product Code. 11540836 Supplier Invitrogen™ Supplier No. V49320

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Description

Includes

pAd-CMV/V5-DEST Vector 150ng/μL in TEBuffer, pH 8.0. 40μL; pAd/CMV/V5-GW/lacZ control plasmid 1μg/μL in TE Buffer, pH 8.0, 10μL.

For easy recombination-based cloning and adenoviral-based, transient expression of a target gene in dividing and nondividing mammalian cells

  • The vector allows generation of an adenovirus containing the target gene where constitutive, high-level expression is driven by the CMV promoter
  • High efficiency and rapid recombination cloning
  • Produces high titer adenoviral stocks
  • Efficient delivery of the gene to dividing and nondividing mammalian cells in vitro or in vivo
  • Produces replication-incompetent virus for enhanced biosafety of the system
  • Amenable for use in high-throughput applications
  • CMV promoter for high-level constitutive expression of gene of interest
  • Human Ad5 sequences (ΔE3) and Viral Inverted Terminal Repeats (ITRs) for packaging of the expression construct into virions
  • Herpes Simplex Virus thymidine kinase (TK) polyadenylation sequence for efficient transcription termination and polyadenylation of mRNA
  • V5 epitope for detection of recombinant protein
  • Ampicillin selection marker

Antibody and Cell Culture Production, Bioproduction, Cloning, Constitutive Expression, Gateway Cloning, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Viral Gene Delivery, Viral Vaccine Production

TRUSTED_SUSTAINABILITY

Specifications

Constitutive or Inducible System Constitutive
Delivery Type Adenoviral
Promoter CMV
Product Type Mammalian Expression Vector
Content And Storage • pAd-CMV⁄V5-DEST™ Vector 150 ng⁄μl in TEBuffer, pH 8.0. 40 μl (Store at -20°C)
• pAd⁄CMV⁄V5-GW⁄lacZ control plasmid 1 μg⁄μl in TE Buffer, pH 8.0. 10 μl (Store at -20°C)
Protein Tag V5 Epitope Tag
Cloning Method Gateway
Quantity 6 μg
Vector pDEST
Product Line Gateway, ViraPower
For Use With (Application) Viral Expression
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For Research Use Only. Not for use in diagnostic procedures.

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