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Invitrogen™ Gateway™ pDONR™221 Vector
Description
Includes
6μg pDONR221 vector, lyophilized in TE buffer, pH 8.0.
- Designed to create entry clone following PCR with attB-modified custom primers
- With entry clone, recombine gene of interest into variety of expression vectors adapted for use in Gateway technology
- PCR product is directionally cloned with efficiencies typically >99%
- Rapid, efficient, directional cloning of PCR products
- Versatility to use any amplification enzyme
- Cloning without need for restriction endonucleases or ligase
- Selection of entry clones with kanamycin or Zeocin™
- In addition, pDONR221 and pDONR™/Zeo vectors have pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use
Cloning, Gateway Cloning
Specifications
Specifications
| Product Type | Gateway System Destination Expression Vector |
| Content And Storage | 6 μg pDONR221™ vector, lyophilized in TE buffer, pH 8.0. Upon receipt, store at -20°C. |
| Cloning Method | Gateway |
| Quantity | 6 μg |
| Vector | pDONR |
| Product Line | Gateway, pDONR |
For Research Use Only. Not for use in diagnostic procedures.
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