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Invitrogen™ pTrcHis2 A, B, & C Bacterial Expression Vectors

Product Code. 10554383 Shop All Thermo Scientific Products
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Quantity:
20 μg
Unit Size:
Each
This item is not returnable. View return policy

Product Code. 10554383

Brand: Invitrogen™ V36520

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This item is not returnable. View return policy

Description

Includes

All vectors are supercoiled and are provided lyophilized. A TOP10 E. coli stab and positive expression control are provided.

Offer enhanced translation initiation and high-level expression in E. coli

  • These vectors feature high-level regulated transcription from the trc promoter
  • Enhanced translation efficiency of eukaryotic genes in E. coli
  • The lacO operator and lacIq repressor gene for transcriptional regulation in any E. coli strain
  • This particular vector offers C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an anti-HisG antibody
  • C-terminal Xpress epitope for easy detection with an anti-Xpress antibody
  • Enterokinase cleavage site for removal of fusion tag

Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

TRUSTED_SUSTAINABILITY

Specifications

Constitutive or Inducible System Inducible
Inducing Agent IPTG
Promoter Trc, lacO
Product Type Bacterial Expression Vector
Selection Agent (Eukaryotic) None
Content And Storage All vectors are supercoiled and are provided lyophilized. A TOP10 E. coli stab and positive expression control are provided. Store vectors at -20°C. Store stab at room temperature. The vector is guaranteed stable for 6 months when properly stored.
Antibiotic Resistance Bacterial Ampicillin (AmpR)
Cleavage EK (Enterokinase) Recognition Site
Protein Tag Thioredoxin
Cloning Method Restriction Enzyme/MCS
Quantity 20 μg
Vector pTrc
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For Research Use Only. Not for use in diagnostic procedures.

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