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Invitrogen™ ViraPower™ II Lentiviral Gateway™ Expression System
Description
Includes
6μg of pLenti6.2/V5-DEST vector (40μL vector at 150ng/μL in TE Buffer, pH 8.0), 10μg of pLenti6.2/V5-GW/lacZ plasmid (20μL of plasmid at 0.5μg/μL in TE Buffer, pH 8.0), Stbl3 Chemically Competent E. coli; 21 x 50μL of Stbl3 Cells, 6mL of S.O.C.Medium and 50μL of pUC19 control DNA (10pg/μL); ViraPower Bsd Lentiviral Support Kit includes 195μg of ViraPower Packaging Mix (Contains 195μL of a mixture of pLP1, pLP2, and pLP/VSVG plasmids, at 1μg/μL in TE Buffer, pH 8.0), 0.75mL of Lipofectamine 2000, 50mg of Blasticidin powder; 293FT Cell Line, 1 vial containing 3 X 106frozen cells in 1mL of Freezing Medium
- Generates replication-incompetent lentivirus for transducing dividing and nondividing mammalian cells
- The pLenti6.2/V5-DESTvector in kit has CMV promoter for driving constitutive expression of target gene in mammalian cells
- Uses the PGK promoter instead of SV40 promoter in lentiviral vector for driving long-term, persistent expression of blasticidin stable selection marker
- Easy recombination-based cloning and stable expression, long term expression
- Blasticidin selection marker for stable selection under control of PGK promoter for long-term, persistent expression
Cloning, Constitutive Expression, Gateway Cloning, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Viral Gene Delivery
Specifications
Specifications
| Constitutive or Inducible System | Constitutive |
| Delivery Type | Lentiviral |
| Promoter | CMV |
| Product Type | Lentiviral Expression System |
| Selection Agent (Eukaryotic) | Blasticidin |
| Content And Storage | pLenti6.2⁄V5-DEST™ Gateway™ Vector Kit • 6 μg of pLenti6.2⁄V5-DEST™ vector (40 μLofvector at 150 ng⁄μL in TE Buffer, pH 8.0) • 10 μg of pLenti6.2⁄V5-GW⁄lacZ™ plasmid(20μL of plasmid at 0.5 μg⁄μL in TE Buffer, pH 8.0) • One Shot™ Stbl3™ Chemically Competent E. coli(Cat # C737303): 21 x 50-μL of Stbl3™ Cells, 6 mL of S.O.C.Medium and 50 μL of pUC19 control DNA (10 pg⁄μL). ViraPower™ Bsd Lentiviral Support Kit (Cat # K497000) • 195 μg of ViraPower™ Packaging Mix (Contains 195μL of a mixture of pLP1, pLP2, and pLP⁄VSVG plasmids, at 1μg⁄μL in TE Buffer, pH 8.0) • 0.75 ml of Lipofectamine™ 2000 (Cat # 11668027) • 50 mg of Blasticidin powder (Cat # R21001) • 293FT Cell Line (Cat # R70007): 1 vial containing 3 X 106frozen cells in 1 mL of Freezing Medium. Storage • Vectors: -20°C • ViraPower™ Packaging Mix: -20°C • Blasticidin: -20°C • Lipofectamine™ 2000: 4°C (do not freeze) • One Shot™ Stbl3™ Chemically Competent E. coli:-80°C • 293 FT Cells: Liquid Nitrogen |
| Protein Tag | V5 Epitope Tag |
| Cloning Method | Gateway |
| Quantity | 1 kit |
| Vector | pLenti |
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For Research Use Only. Not for use in diagnostic procedures.
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